Use of PCR for the determination of the frequency of the deltaF508 mutation in Brasilian cistic fibrosis patients

Use of PCR for the determination of the frequency of the deltaF508 mutation in Brasilian cistic fibrosis patients

The [Delta]F508 mutation in the cystic fibrosis (CF) gene was studied in a population of 18 Brazilian CF patients and their 17 families by use of PCR and differential hybridization with oligonucleotides. In a total of 34 chromosomes considered, 12 (35%) carried the F508 deletion, a frequency much lo...

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Bibliographic Details
Main Authors: Antonio B. de Miranda, Juan LLerena Junior, Ludma T. Dallalana, Rodrigo S. Moura-Neto, Philip N. Suffys, Wim M. Degrave
Format: Article
Language:English
Published: Fundação Oswaldo Cruz (FIOCRUZ) 1993-06-01
Series:Memorias do Instituto Oswaldo Cruz
Subjects:
cystic fibrosis
PCR
[Delta]F508
diagnosis
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761993000200022

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761993000200022

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