One-step CRISPR-Cas9-mediated knockout of native TCRαβ genes in human T cells using RNA electroporation
Summary: To avoid mispairing between native and introduced T cell receptors (TCRs) and to prevent graft-versus-host disease in allogeneic T cell therapies, TCRα and TCRβ chains of native TCRs are knocked out via CRISPR-Cas9. We demonstrate the isolation and activation of CD8+ T cells followed by ele...
Main Authors: | , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2023-03-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723000709 |