One-step CRISPR-Cas9-mediated knockout of native TCRαβ genes in human T cells using RNA electroporation

One-step CRISPR-Cas9-mediated knockout of native TCRαβ genes in human T cells using RNA electroporation

Summary: To avoid mispairing between native and introduced T cell receptors (TCRs) and to prevent graft-versus-host disease in allogeneic T cell therapies, TCRα and TCRβ chains of native TCRs are knocked out via CRISPR-Cas9. We demonstrate the isolation and activation of CD8+ T cells followed by ele...

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Bibliographic Details
Main Authors: Donovan Flumens, Diana Campillo-Davo, Ibo Janssens, Gils Roex, Jorrit De Waele, Sébastien Anguille, Eva Lion
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Cell culture
Cell isolation
Flow Cytometry/Mass Cytometry
Immunology
CRISPR
Tissue Engineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723000709

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