Cloning and expression of the sucrose phosphorylase gene in Bacillus subtilis and synthesis of kojibiose using the recombinant enzyme

Abstract Background Kojibiose as a prebiotic and inhibitor of α-glucosidase exhibits potential for a wide range of applications in the food and medicine fields; however, large-scale separation and extraction of kojibiose from nature is difficult. Sucrose phosphorylase (SPase) can be used for the pro...

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Bibliographic Details
Main Authors: Miaomiao Wang, Jing Wu, Dan Wu
Format: Article
Language:English
Published: BMC 2018-02-01
Series:Microbial Cell Factories
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12934-017-0842-2