Separation and Paired Proteome Profiling of Plant Chloroplast and Cytoplasmic Ribosomes

Separation and Paired Proteome Profiling of Plant Chloroplast and Cytoplasmic Ribosomes

Conventional preparation methods of plant ribosomes fail to resolve non-translating chloroplast or cytoplasmic ribosome subunits from translating fractions. We established preparation of these ribosome complexes from <i>Arabidopsis thaliana</i> leaf, root, and seed tissues by optimized s...

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Bibliographic Details
Main Authors: Alexandre Augusto Pereira Firmino, Michal Gorka, Alexander Graf, Aleksandra Skirycz, Federico Martinez-Seidel, Kerstin Zander, Joachim Kopka, Olga Beine-Golovchuk
Format: Article
Language:English
Published: MDPI AG 2020-07-01
Series:Plants
Subjects:
in vivo protein complex stabilization
plant proteomics
ribo-proteome
ribosome complexes
tissue specific separation
Online Access:https://www.mdpi.com/2223-7747/9/7/892

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https://www.mdpi.com/2223-7747/9/7/892

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