Combined Multiplexed Phage Display, High-Throughput Sequencing, and Functional Assays as a Platform for Identifying Modulatory VHHs Targeting the FSHR

Combined Multiplexed Phage Display, High-Throughput Sequencing, and Functional Assays as a Platform for Identifying Modulatory VHHs Targeting the FSHR

Developing modulatory antibodies against G protein-coupled receptors is challenging. In this study, we targeted the follicle-stimulating hormone receptor (FSHR), a significant regulator of reproduction, with variable domains of heavy chain-only antibodies (VHHs). We built two immune VHH libraries an...

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Bibliographic Details
Main Authors: Anielka Zehnaker, Amandine Vallet, Juliette Gourdon, Caterina Sarti, Vinesh Jugnarain, Maya Haj Hassan, Laetitia Mathias, Camille Gauthier, Pauline Raynaud, Thomas Boulo, Linda Beauclair, Yves Bigot, Livio Casarini, Pascale Crépieux, Anne Poupon, Benoît Piégu, Frédéric Jean-Alphonse, Gilles Bruneau, Éric Reiter
Format: Article
Language:English
Published: MDPI AG 2023-11-01
Series:International Journal of Molecular Sciences
Subjects:
phage display
VHH
GPCR
FSHR
next-generation sequencing
Online Access:https://www.mdpi.com/1422-0067/24/21/15961

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https://www.mdpi.com/1422-0067/24/21/15961

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