Stable production of recombinant SARS-CoV-2 receptor-binding domain in mammalian cells with co-expression of a fluorescent reporter and its validation as antigenic target for COVID-19 serology testing

Stable production of recombinant SARS-CoV-2 receptor-binding domain in mammalian cells with co-expression of a fluorescent reporter and its validation as antigenic target for COVID-19 serology testing

SARS-CoV-2 receptor binding domain (RBD) recognizes the angiotensin converting enzyme 2 (ACE2) receptor in host cells that enables infection. Due to its antigenic specificity, RBD production is important for development of serological assays. Here we have established a system for stable RBD producti...

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Bibliographic Details
Main Authors: Jorge L. Arias-Arias, Silvia E. Molina-Castro, Laura Monturiol-Gross, Bruno Lomonte, Eugenia Corrales-Aguilar
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:Biotechnology Reports
Subjects:
SARS-CoV-2
Recombinant RBD expression
Fluorescent protein
Mammalian cells
COVID-19 serology
Online Access:http://www.sciencedirect.com/science/article/pii/S2215017X22000777

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http://www.sciencedirect.com/science/article/pii/S2215017X22000777

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