SMRT Sequencing Enables High-Throughput Identification of Novel AAVs from Capsid Shuffling and Directed Evolution

SMRT Sequencing Enables High-Throughput Identification of Novel AAVs from Capsid Shuffling and Directed Evolution

The use of AAV capsid libraries coupled with various selection strategies has proven to be a remarkable approach for generating novel AAVs with enhanced and desired features. The inability to reliably sequence the complete capsid gene in a high-throughput manner has been the bottleneck of capsid eng...

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Bibliographic Details
Main Authors: Widler Casy, Irvin T. Garza, Xin Chen, Thomas Dong, Yuhui Hu, Mohammed Kanchwala, Cynthia B. Trygg, Charles Shyng, Chao Xing, Bruce A. Bunnell, Stephen E. Braun, Steven J. Gray
Format: Article
Language:English
Published: MDPI AG 2023-08-01
Series:Genes
Subjects:
AAV
capsid engineering
capsid shuffling
directed evolution
SMRT sequencing
vector development
Online Access:https://www.mdpi.com/2073-4425/14/8/1660

Internet

https://www.mdpi.com/2073-4425/14/8/1660

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