Quantification of membrane-bound cytokine receptors by calibrated flow cytometry
Summary: We present a protocol for quantifying the expression of the receptor gp130 using a calibrated flow cytometric approach. We describe pitfalls for receptor quantification such as titration of primary antibodies and standardizing cell culture. Receptors are stained with primary antibodies and...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2023-09-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723004781 |