The influences of morphine or ketamine pre-treatment on hemodynamic, acid-base status, biochemical markers of brain damage and early survival in rats after asphyxial cardiac arrest
Abstract Background In different models of hypoxia, blockade of opioid or N-methyl-D-aspartate (NMDA) receptors shows cardio- and neuroprotective effects with a consequent increase in animal survival. The aim of the study was to investigate effects of pre-treatment with Morphine or Ketamine on hemod...
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| Language: | English |
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BMC
2019-11-01
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| Series: | BMC Anesthesiology |
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| Online Access: | http://link.springer.com/article/10.1186/s12871-019-0884-6 |
| _version_ | 1818340913264459776 |
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| author | Vladimir Kuklin Nurlan Akhatov Timofei Kondratiev Aidos Konkayev Abai Baigenzhin Maiya Konkayeva Temirlan Karibekov Nicholas Barlow Torkjel Tveita Vegard Dahl |
| author_facet | Vladimir Kuklin Nurlan Akhatov Timofei Kondratiev Aidos Konkayev Abai Baigenzhin Maiya Konkayeva Temirlan Karibekov Nicholas Barlow Torkjel Tveita Vegard Dahl |
| author_sort | Vladimir Kuklin |
| collection | DOAJ |
| description | Abstract Background In different models of hypoxia, blockade of opioid or N-methyl-D-aspartate (NMDA) receptors shows cardio- and neuroprotective effects with a consequent increase in animal survival. The aim of the study was to investigate effects of pre-treatment with Morphine or Ketamine on hemodynamic, acid-base status, early survival, and biochemical markers of brain damage in a rat model of asphyxial cardiac arrest (ACA). Methods Under anaesthesia with Thiopental Sodium 60 mg/kg, i.p., Wistar rats (n = 42) were tracheostomized and catheters were inserted in a femoral vein and artery. After randomization, the rats were pre-treated with: Morphine 5 mg/kg i.v. (n = 14); Ketamine 40 mg/kg i.v. (n = 14); or equal volume of i.v. NaCl 0.9% as a Control (n = 14). ACA was induced by corking of the tracheal tube for 8 min, and defined as a mean arterial pressure (MAP) < 20 mmHg. Resuscitation was started at 5 min after cardiac arrest (CA). Invasive MAP was recorded during experiments. Arterial pH and blood gases were sampled at baseline (BL) and 10 min after CA. At the end of experiments, all surviving rats were euthanised, brain and blood samples for measurement of Neuron Specific Enolase (NSE), s100 calcium binding protein B (s100B) and Caspase-3 (CS-3) were retrieved. Results At BL no differences between groups were found in hemodynamic or acid-base status. After 3 min of asphyxia, all animals had cardiac arrest (CA). Return of spontaneous circulation (MAP > 60 mmHg) was achieved in all animals within 3 min after CA. At the end of the experiment, the Ketamine pre-treated group had increased survival (13 of 14; 93%) compared to the Control (7 of 14; 50%) and Morphine (10 of 14; 72%) groups (p = 0.035). Biochemical analysis of plasma concentration of NSE and s100B as well as an analysis of CS-3 levels in the brain tissue did not reveal any differences between the study groups. Conclusion In rats after ACA, pre-treatment with Morphine or Ketamine did not have any significant influence on hemodynamic and biochemical markers of brain damage. However, significantly better pH level and increased early survival were found in the Ketamine pre-treated group. |
| first_indexed | 2024-12-13T15:50:27Z |
| format | Article |
| id | doaj.art-810ea3120689498db997f360376822dc |
| institution | Directory Open Access Journal |
| issn | 1471-2253 |
| language | English |
| last_indexed | 2024-12-13T15:50:27Z |
| publishDate | 2019-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Anesthesiology |
| spelling | doaj.art-810ea3120689498db997f360376822dc2022-12-21T23:39:31ZengBMCBMC Anesthesiology1471-22532019-11-011911910.1186/s12871-019-0884-6The influences of morphine or ketamine pre-treatment on hemodynamic, acid-base status, biochemical markers of brain damage and early survival in rats after asphyxial cardiac arrestVladimir Kuklin0Nurlan Akhatov1Timofei Kondratiev2Aidos Konkayev3Abai Baigenzhin4Maiya Konkayeva5Temirlan Karibekov6Nicholas Barlow7Torkjel Tveita8Vegard Dahl9Department of Anaesthesiology and Intensive Care Medicine, Akershus university hospitalDepartment of Anaesthesiology and Intensive Care Medicine, Astana Medical UniversityAnaesthesia and Critical Care Research Group, Department of Clinical Medicine, UiT – The Arctic University of NorwayDepartment of Anaesthesiology and Intensive Care Medicine, Astana Medical UniversityDepartment of Anaesthesiology and Intensive Care Medicine, National Scientific Medical CenterDepartment of Anaesthesiology and Intensive Care Medicine, Astana Medical UniversityDepartment of Anaesthesiology and Intensive Care Medicine, National Scientific Medical CenterDepartment of Anaesthesiology and Intensive Care Medicine, Akershus university hospitalDivision of Surgical Medicine and Intensive Care, University Hospital of Northern NorwayDepartment of Anaesthesiology and Intensive Care Medicine, Akershus university hospitalAbstract Background In different models of hypoxia, blockade of opioid or N-methyl-D-aspartate (NMDA) receptors shows cardio- and neuroprotective effects with a consequent increase in animal survival. The aim of the study was to investigate effects of pre-treatment with Morphine or Ketamine on hemodynamic, acid-base status, early survival, and biochemical markers of brain damage in a rat model of asphyxial cardiac arrest (ACA). Methods Under anaesthesia with Thiopental Sodium 60 mg/kg, i.p., Wistar rats (n = 42) were tracheostomized and catheters were inserted in a femoral vein and artery. After randomization, the rats were pre-treated with: Morphine 5 mg/kg i.v. (n = 14); Ketamine 40 mg/kg i.v. (n = 14); or equal volume of i.v. NaCl 0.9% as a Control (n = 14). ACA was induced by corking of the tracheal tube for 8 min, and defined as a mean arterial pressure (MAP) < 20 mmHg. Resuscitation was started at 5 min after cardiac arrest (CA). Invasive MAP was recorded during experiments. Arterial pH and blood gases were sampled at baseline (BL) and 10 min after CA. At the end of experiments, all surviving rats were euthanised, brain and blood samples for measurement of Neuron Specific Enolase (NSE), s100 calcium binding protein B (s100B) and Caspase-3 (CS-3) were retrieved. Results At BL no differences between groups were found in hemodynamic or acid-base status. After 3 min of asphyxia, all animals had cardiac arrest (CA). Return of spontaneous circulation (MAP > 60 mmHg) was achieved in all animals within 3 min after CA. At the end of the experiment, the Ketamine pre-treated group had increased survival (13 of 14; 93%) compared to the Control (7 of 14; 50%) and Morphine (10 of 14; 72%) groups (p = 0.035). Biochemical analysis of plasma concentration of NSE and s100B as well as an analysis of CS-3 levels in the brain tissue did not reveal any differences between the study groups. Conclusion In rats after ACA, pre-treatment with Morphine or Ketamine did not have any significant influence on hemodynamic and biochemical markers of brain damage. However, significantly better pH level and increased early survival were found in the Ketamine pre-treated group.http://link.springer.com/article/10.1186/s12871-019-0884-6MorphineKetamineRatsAsphyxial cardiac arrestEarly survival |
| spellingShingle | Vladimir Kuklin Nurlan Akhatov Timofei Kondratiev Aidos Konkayev Abai Baigenzhin Maiya Konkayeva Temirlan Karibekov Nicholas Barlow Torkjel Tveita Vegard Dahl The influences of morphine or ketamine pre-treatment on hemodynamic, acid-base status, biochemical markers of brain damage and early survival in rats after asphyxial cardiac arrest BMC Anesthesiology Morphine Ketamine Rats Asphyxial cardiac arrest Early survival |
| title | The influences of morphine or ketamine pre-treatment on hemodynamic, acid-base status, biochemical markers of brain damage and early survival in rats after asphyxial cardiac arrest |
| title_full | The influences of morphine or ketamine pre-treatment on hemodynamic, acid-base status, biochemical markers of brain damage and early survival in rats after asphyxial cardiac arrest |
| title_fullStr | The influences of morphine or ketamine pre-treatment on hemodynamic, acid-base status, biochemical markers of brain damage and early survival in rats after asphyxial cardiac arrest |
| title_full_unstemmed | The influences of morphine or ketamine pre-treatment on hemodynamic, acid-base status, biochemical markers of brain damage and early survival in rats after asphyxial cardiac arrest |
| title_short | The influences of morphine or ketamine pre-treatment on hemodynamic, acid-base status, biochemical markers of brain damage and early survival in rats after asphyxial cardiac arrest |
| title_sort | influences of morphine or ketamine pre treatment on hemodynamic acid base status biochemical markers of brain damage and early survival in rats after asphyxial cardiac arrest |
| topic | Morphine Ketamine Rats Asphyxial cardiac arrest Early survival |
| url | http://link.springer.com/article/10.1186/s12871-019-0884-6 |
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