High-content screening of mitochondrial polarization in neural cells derived from human pluripotent stem cells

High-content screening of mitochondrial polarization in neural cells derived from human pluripotent stem cells

Summary: We present a high-content screening (HCS) protocol for quantifying mitochondrial activity in live neural cells from human induced pluripotent stem cells (iPSCs). The assessment is based on mitochondrial membrane potential, which is influenced by the efficiency of mitochondrial bioenergetics...

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Bibliographic Details
Main Authors: Annika Zink, Undine Haferkamp, Annika Wittich, Mathias Beller, Ole Pless, Alessandro Prigione
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell-based Assays
Metabolism
Microscopy
Neuroscience
Stem Cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722004828

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http://www.sciencedirect.com/science/article/pii/S2666166722004828

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