Optimization of Plasma Activated Water Extraction of <i>Pleurotus ostreatus</i> Polysaccharides on Its Physiochemical and Biological Activity Using Response Surface Methodology
This study focused on optimizing the extraction of <i>P. ostreatus</i> polysaccharides (POPs) using plasma-activated water (PAW). A single factor and response surface methodology were employed to optimize and evaluate the polysaccharide yield, physiochemical characteristics, and biologic...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/23/4347 |
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| author | Fuangfah Punthi Bara Yudhistira Mohsen Gavahian Chao-Kai Chang Naila Husnayain Chih-Yao Hou Cheng-Chia Yu Chang-Wei Hsieh |
| author_facet | Fuangfah Punthi Bara Yudhistira Mohsen Gavahian Chao-Kai Chang Naila Husnayain Chih-Yao Hou Cheng-Chia Yu Chang-Wei Hsieh |
| author_sort | Fuangfah Punthi |
| collection | DOAJ |
| description | This study focused on optimizing the extraction of <i>P. ostreatus</i> polysaccharides (POPs) using plasma-activated water (PAW). A single factor and response surface methodology were employed to optimize and evaluate the polysaccharide yield, physiochemical characteristics, and biological activities of POPs. The observed findings were compared to those obtained by the conventional hot water extraction method (100 °C, 3 h), as the control treatment. The optimal extraction conditions were obtained at 700 W PAW power, 58 s treatment time, 1:19 sample-to-water ratio, and 15 L/min gas flow rate. In these conditions, the PAW-treated samples experienced changes in surface morphology due to plasma etching, leading to a 288% increase in the polysaccharide yield (11.67%) compared to the control sample (3.01%). Furthermore, the PAW-treated sample exhibited superior performance in terms of biological activities, namely phenolic compounds (53.79 mg GAE/100 g), DPPH scavenging activity (72.77%), and OH scavenging activity (65.03%), which were 29%, 18%, and 38% higher than those of control sample, respectively. The results highlighted the importance of process optimization and provided new evidence for PAW as an alternative approach to enhance the extraction efficiency of POPs, a novel source of natural antioxidants which enables diverse applications in the food industry. |
| first_indexed | 2024-03-09T01:50:54Z |
| format | Article |
| id | doaj.art-81288600e962455eb9a609f7bbfb2ea4 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T01:50:54Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-81288600e962455eb9a609f7bbfb2ea42023-12-08T15:15:50ZengMDPI AGFoods2304-81582023-12-011223434710.3390/foods12234347Optimization of Plasma Activated Water Extraction of <i>Pleurotus ostreatus</i> Polysaccharides on Its Physiochemical and Biological Activity Using Response Surface MethodologyFuangfah Punthi0Bara Yudhistira1Mohsen Gavahian2Chao-Kai Chang3Naila Husnayain4Chih-Yao Hou5Cheng-Chia Yu6Chang-Wei Hsieh7Department of Food Science and Biotechnology, National Chung Hsing University, Taichung City 40227, TaiwanDepartment of Food Science and Biotechnology, National Chung Hsing University, Taichung City 40227, TaiwanDepartment of Food Science, National Pingtung University of Science and Technology, Pingtung City 91201, TaiwanDepartment of Food Science and Biotechnology, National Chung Hsing University, Taichung City 40227, TaiwanInternational Master Program of Agriculture, National Chung Hsing University, Taichung City 40227, TaiwanDepartment of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, TaiwanInstitute of Oral Sciences, Chung Shan Medical University, Taichung City 40201, TaiwanDepartment of Food Science and Biotechnology, National Chung Hsing University, Taichung City 40227, TaiwanThis study focused on optimizing the extraction of <i>P. ostreatus</i> polysaccharides (POPs) using plasma-activated water (PAW). A single factor and response surface methodology were employed to optimize and evaluate the polysaccharide yield, physiochemical characteristics, and biological activities of POPs. The observed findings were compared to those obtained by the conventional hot water extraction method (100 °C, 3 h), as the control treatment. The optimal extraction conditions were obtained at 700 W PAW power, 58 s treatment time, 1:19 sample-to-water ratio, and 15 L/min gas flow rate. In these conditions, the PAW-treated samples experienced changes in surface morphology due to plasma etching, leading to a 288% increase in the polysaccharide yield (11.67%) compared to the control sample (3.01%). Furthermore, the PAW-treated sample exhibited superior performance in terms of biological activities, namely phenolic compounds (53.79 mg GAE/100 g), DPPH scavenging activity (72.77%), and OH scavenging activity (65.03%), which were 29%, 18%, and 38% higher than those of control sample, respectively. The results highlighted the importance of process optimization and provided new evidence for PAW as an alternative approach to enhance the extraction efficiency of POPs, a novel source of natural antioxidants which enables diverse applications in the food industry.https://www.mdpi.com/2304-8158/12/23/4347<i>Pleurotus ostreatus</i>plasma-activated waterextractionpolysaccharidesantioxidant activityresponse surface methodology |
| spellingShingle | Fuangfah Punthi Bara Yudhistira Mohsen Gavahian Chao-Kai Chang Naila Husnayain Chih-Yao Hou Cheng-Chia Yu Chang-Wei Hsieh Optimization of Plasma Activated Water Extraction of <i>Pleurotus ostreatus</i> Polysaccharides on Its Physiochemical and Biological Activity Using Response Surface Methodology Foods <i>Pleurotus ostreatus</i> plasma-activated water extraction polysaccharides antioxidant activity response surface methodology |
| title | Optimization of Plasma Activated Water Extraction of <i>Pleurotus ostreatus</i> Polysaccharides on Its Physiochemical and Biological Activity Using Response Surface Methodology |
| title_full | Optimization of Plasma Activated Water Extraction of <i>Pleurotus ostreatus</i> Polysaccharides on Its Physiochemical and Biological Activity Using Response Surface Methodology |
| title_fullStr | Optimization of Plasma Activated Water Extraction of <i>Pleurotus ostreatus</i> Polysaccharides on Its Physiochemical and Biological Activity Using Response Surface Methodology |
| title_full_unstemmed | Optimization of Plasma Activated Water Extraction of <i>Pleurotus ostreatus</i> Polysaccharides on Its Physiochemical and Biological Activity Using Response Surface Methodology |
| title_short | Optimization of Plasma Activated Water Extraction of <i>Pleurotus ostreatus</i> Polysaccharides on Its Physiochemical and Biological Activity Using Response Surface Methodology |
| title_sort | optimization of plasma activated water extraction of i pleurotus ostreatus i polysaccharides on its physiochemical and biological activity using response surface methodology |
| topic | <i>Pleurotus ostreatus</i> plasma-activated water extraction polysaccharides antioxidant activity response surface methodology |
| url | https://www.mdpi.com/2304-8158/12/23/4347 |
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