Postnatal nutrition environment reprograms renal DNA methylation patterns in offspring of maternal protein-restricted stroke-prone spontaneously hypertensive rats
Maternal malnutrition hampers the offspring health by manipulating the epigenome. Recent studies indicate that the changes in DNA methylation could be reversed by afterbirth nutrition supplementation. In this study, we used DNA methylation arrays to comprehensively investigate the DNA methylation st...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2023.1134955/full |
| _version_ | 1797847708764995584 |
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| author | Chika Ando Sihui Ma Sihui Ma Moe Miyoshi Kyohei Furukawa Kyohei Furukawa Xuguang Li Huijuan Jia Hisanori Kato |
| author_facet | Chika Ando Sihui Ma Sihui Ma Moe Miyoshi Kyohei Furukawa Kyohei Furukawa Xuguang Li Huijuan Jia Hisanori Kato |
| author_sort | Chika Ando |
| collection | DOAJ |
| description | Maternal malnutrition hampers the offspring health by manipulating the epigenome. Recent studies indicate that the changes in DNA methylation could be reversed by afterbirth nutrition supplementation. In this study, we used DNA methylation arrays to comprehensively investigate the DNA methylation status of the renal promoter regions and the effects of postnatal protein intake on DNA methylation. We fed stroke-prone spontaneously hypertensive (SHRSP) rat dams a normal diet or a low-protein diet during pregnancy, and their 4-week-old male offspring were fed a normal diet or a high−/low-protein diet for 2 weeks. We found that the methylation status of 2,395 differentially methylated DNA regions was reprogrammed, and 34 genes were reset by different levels of postnatal protein intake in the offspring. Among these genes, Adora2b, Trpc5, Ar, Xrcc2, and Atp1b1 are involved in renal disease and blood pressure regulation. Our findings indicate that postnatal nutritional interventions can potentially reprogram epigenetic changes, providing novel therapeutic and preventive epigenetic targets for salt-sensitive hypertension. |
| first_indexed | 2024-04-09T18:16:43Z |
| format | Article |
| id | doaj.art-8144862127104a56add4715d6ba0c705 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-09T18:16:43Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-8144862127104a56add4715d6ba0c7052023-04-13T04:26:02ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2023-04-011010.3389/fnut.2023.11349551134955Postnatal nutrition environment reprograms renal DNA methylation patterns in offspring of maternal protein-restricted stroke-prone spontaneously hypertensive ratsChika Ando0Sihui Ma1Sihui Ma2Moe Miyoshi3Kyohei Furukawa4Kyohei Furukawa5Xuguang Li6Huijuan Jia7Hisanori Kato8Health Nutrition, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, JapanHealth Nutrition, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, JapanFaculty of Sport Sciences, Waseda University, Tokorozawa, JapanHealth Nutrition, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, JapanHealth Nutrition, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, JapanAnimal Nutrition, Life Sciences, Graduate School of Agricultural Science, Tohoku University, Sendai, JapanHealth Nutrition, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, JapanHealth Nutrition, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, JapanHealth Nutrition, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, JapanMaternal malnutrition hampers the offspring health by manipulating the epigenome. Recent studies indicate that the changes in DNA methylation could be reversed by afterbirth nutrition supplementation. In this study, we used DNA methylation arrays to comprehensively investigate the DNA methylation status of the renal promoter regions and the effects of postnatal protein intake on DNA methylation. We fed stroke-prone spontaneously hypertensive (SHRSP) rat dams a normal diet or a low-protein diet during pregnancy, and their 4-week-old male offspring were fed a normal diet or a high−/low-protein diet for 2 weeks. We found that the methylation status of 2,395 differentially methylated DNA regions was reprogrammed, and 34 genes were reset by different levels of postnatal protein intake in the offspring. Among these genes, Adora2b, Trpc5, Ar, Xrcc2, and Atp1b1 are involved in renal disease and blood pressure regulation. Our findings indicate that postnatal nutritional interventions can potentially reprogram epigenetic changes, providing novel therapeutic and preventive epigenetic targets for salt-sensitive hypertension.https://www.frontiersin.org/articles/10.3389/fnut.2023.1134955/fullDNA methylationepigeneticsgene expressionhypertensionkidneymaternal protein restriction |
| spellingShingle | Chika Ando Sihui Ma Sihui Ma Moe Miyoshi Kyohei Furukawa Kyohei Furukawa Xuguang Li Huijuan Jia Hisanori Kato Postnatal nutrition environment reprograms renal DNA methylation patterns in offspring of maternal protein-restricted stroke-prone spontaneously hypertensive rats Frontiers in Nutrition DNA methylation epigenetics gene expression hypertension kidney maternal protein restriction |
| title | Postnatal nutrition environment reprograms renal DNA methylation patterns in offspring of maternal protein-restricted stroke-prone spontaneously hypertensive rats |
| title_full | Postnatal nutrition environment reprograms renal DNA methylation patterns in offspring of maternal protein-restricted stroke-prone spontaneously hypertensive rats |
| title_fullStr | Postnatal nutrition environment reprograms renal DNA methylation patterns in offspring of maternal protein-restricted stroke-prone spontaneously hypertensive rats |
| title_full_unstemmed | Postnatal nutrition environment reprograms renal DNA methylation patterns in offspring of maternal protein-restricted stroke-prone spontaneously hypertensive rats |
| title_short | Postnatal nutrition environment reprograms renal DNA methylation patterns in offspring of maternal protein-restricted stroke-prone spontaneously hypertensive rats |
| title_sort | postnatal nutrition environment reprograms renal dna methylation patterns in offspring of maternal protein restricted stroke prone spontaneously hypertensive rats |
| topic | DNA methylation epigenetics gene expression hypertension kidney maternal protein restriction |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2023.1134955/full |
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