Repurposing Type I-A CRISPR-Cas3 for a robust diagnosis of human papillomavirus (HPV)

Repurposing Type I-A CRISPR-Cas3 for a robust diagnosis of human papillomavirus (HPV)

Abstract R-loop-triggered collateral single-stranded DNA (ssDNA) nuclease activity within Class 1 Type I CRISPR-Cas systems holds immense potential for nucleic acid detection. However, the hyperactive ssDNase activity of Cas3 introduces unwanted noise and false-positive results. In this study, we id...

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Bibliographic Details
Main Authors: Tao Hu, Quanquan Ji, Xinxin Ke, Hufeng Zhou, Senfeng Zhang, Shengsheng Ma, Chenlin Yu, Wenjun Ju, Meiling Lu, Yu Lin, Yangjing Ou, Yingsi Zhou, Yibei Xiao, Chunlong Xu, Chunyi Hu
Format: Article
Language:English
Published: Nature Portfolio 2024-07-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-024-06537-3

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https://doi.org/10.1038/s42003-024-06537-3

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