Improved assembly procedure of viral RNA genomes amplified with Phi29 polymerase from new generation sequencing data

Improved assembly procedure of viral RNA genomes amplified with Phi29 polymerase from new generation sequencing data

BACKGROUND: New sequencing technologies have opened the way to the discovery and the characterization of pathogenic viruses in clinical samples. However, the use of these new methods can require an amplification of viral RNA prior to the sequencing. Among all the available methods, the procedure bas...

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Bibliographic Details
Main Authors: Nicolas Berthet, Stéphane Descorps-Declère, Andriniaina Andy Nkili-Meyong, Emmanuel Nakouné, Antoine Gessain, Jean-Claude Manuguerra, Mirdad Kazanji
Format: Article
Language:English
Published: BMC
Series:Biological Research
Subjects:
RNA viral genome
Next generation sequencing
SPAdes
Assembling genome
Amplification with phi29 polymerase
Online Access:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100039&lng=en&tlng=en

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http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100039&lng=en&tlng=en

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