Antioxidant and Anti-Inflammatory Properties of Quail Yolk Oil via Upregulation of Superoxide Dismutase 1 and Catalase Genes and Downregulation of EIGER and Unpaired 2 Genes in a <i>D. melanogaster</i> Model
Quail egg yolk oil (QEYO) has a rich history of medicinal use, showcasing heightened antioxidant and bioactive properties in our prior studies. This positions QEYO as a promising candidate for therapeutic and cosmetic applications. In this investigation, QEYO was extracted using ethanol/chloroform a...
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MDPI AG
2024-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/13/1/75 |
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| author | Muhammad Sani Ismaila Kamaldeen Olalekan Sanusi Uwaisu Iliyasu Mustapha Umar Imam Karla Georges Venkatesan Sundaram Kegan Romelle Jones |
| author_facet | Muhammad Sani Ismaila Kamaldeen Olalekan Sanusi Uwaisu Iliyasu Mustapha Umar Imam Karla Georges Venkatesan Sundaram Kegan Romelle Jones |
| author_sort | Muhammad Sani Ismaila |
| collection | DOAJ |
| description | Quail egg yolk oil (QEYO) has a rich history of medicinal use, showcasing heightened antioxidant and bioactive properties in our prior studies. This positions QEYO as a promising candidate for therapeutic and cosmetic applications. In this investigation, QEYO was extracted using ethanol/chloroform and 2-propanol/hexane solvents. GC–MS and FTIR analyses quantified 14 major bioactive compounds in the ethanol/chloroform fraction and 12 in the 2-propanol/hexane fraction. Toxicity evaluations in fruit flies, spanning acute, sub chronic, and chronic exposures, revealed no adverse effects. Negative geotaxis assays assessed locomotor activity, while biochemical assays using fly hemolymph gauged antioxidant responses. Real-time PCR revealed the relative expression levels of the antioxidant and anti-inflammatory genes. FTIR spectra indicated diverse functional groups, and the GC–MS results associated bioactive compounds with the regulation of the anti-inflammatory genes <i>EIGER</i> and <i>UPD2</i>. While no significant change in SOD activities was noted, male flies treated with specific QEYO doses exhibited increased catalase activity and total antioxidant capacity, coupled with a significant decrease in their malondialdehyde levels. This study offers valuable insights into the bioactive compounds of QEYO and their potential regulatory roles in gene expression. |
| first_indexed | 2024-03-08T11:06:26Z |
| format | Article |
| id | doaj.art-81a3b57f581d41b6a5af994969aa3758 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-08T11:06:26Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-81a3b57f581d41b6a5af994969aa37582024-01-26T14:41:10ZengMDPI AGAntioxidants2076-39212024-01-011317510.3390/antiox13010075Antioxidant and Anti-Inflammatory Properties of Quail Yolk Oil via Upregulation of Superoxide Dismutase 1 and Catalase Genes and Downregulation of EIGER and Unpaired 2 Genes in a <i>D. melanogaster</i> ModelMuhammad Sani Ismaila0Kamaldeen Olalekan Sanusi1Uwaisu Iliyasu2Mustapha Umar Imam3Karla Georges4Venkatesan Sundaram5Kegan Romelle Jones6Department of Basic Veterinary Sciences, School of Veterinary Medicine, The University of the West Indies, St. Augustine 999183, Trinidad and TobagoCentre for Advanced Medical Research and Training (CAMRET), Usmanu Danfodiyo University, Sokoto 840004, NigeriaDepartment of Pharmacognosy and Drug Development, Faculty of Pharmaceutical Sciences, Kaduna State University, Kaduna 800283, NigeriaCentre for Advanced Medical Research and Training (CAMRET), Usmanu Danfodiyo University, Sokoto 840004, NigeriaDepartment of Basic Veterinary Sciences, School of Veterinary Medicine, The University of the West Indies, St. Augustine 999183, Trinidad and TobagoDepartment of Basic Veterinary Sciences, School of Veterinary Medicine, The University of the West Indies, St. Augustine 999183, Trinidad and TobagoDepartment of Basic Veterinary Sciences, School of Veterinary Medicine, The University of the West Indies, St. Augustine 999183, Trinidad and TobagoQuail egg yolk oil (QEYO) has a rich history of medicinal use, showcasing heightened antioxidant and bioactive properties in our prior studies. This positions QEYO as a promising candidate for therapeutic and cosmetic applications. In this investigation, QEYO was extracted using ethanol/chloroform and 2-propanol/hexane solvents. GC–MS and FTIR analyses quantified 14 major bioactive compounds in the ethanol/chloroform fraction and 12 in the 2-propanol/hexane fraction. Toxicity evaluations in fruit flies, spanning acute, sub chronic, and chronic exposures, revealed no adverse effects. Negative geotaxis assays assessed locomotor activity, while biochemical assays using fly hemolymph gauged antioxidant responses. Real-time PCR revealed the relative expression levels of the antioxidant and anti-inflammatory genes. FTIR spectra indicated diverse functional groups, and the GC–MS results associated bioactive compounds with the regulation of the anti-inflammatory genes <i>EIGER</i> and <i>UPD2</i>. While no significant change in SOD activities was noted, male flies treated with specific QEYO doses exhibited increased catalase activity and total antioxidant capacity, coupled with a significant decrease in their malondialdehyde levels. This study offers valuable insights into the bioactive compounds of QEYO and their potential regulatory roles in gene expression.https://www.mdpi.com/2076-3921/13/1/75antioxidant effects<i>EIGER</i> and <i>UPD2</i> gene down-regulationfruit flyFTIRGC–MSquail egg oil extracts |
| spellingShingle | Muhammad Sani Ismaila Kamaldeen Olalekan Sanusi Uwaisu Iliyasu Mustapha Umar Imam Karla Georges Venkatesan Sundaram Kegan Romelle Jones Antioxidant and Anti-Inflammatory Properties of Quail Yolk Oil via Upregulation of Superoxide Dismutase 1 and Catalase Genes and Downregulation of EIGER and Unpaired 2 Genes in a <i>D. melanogaster</i> Model Antioxidants antioxidant effects <i>EIGER</i> and <i>UPD2</i> gene down-regulation fruit fly FTIR GC–MS quail egg oil extracts |
| title | Antioxidant and Anti-Inflammatory Properties of Quail Yolk Oil via Upregulation of Superoxide Dismutase 1 and Catalase Genes and Downregulation of EIGER and Unpaired 2 Genes in a <i>D. melanogaster</i> Model |
| title_full | Antioxidant and Anti-Inflammatory Properties of Quail Yolk Oil via Upregulation of Superoxide Dismutase 1 and Catalase Genes and Downregulation of EIGER and Unpaired 2 Genes in a <i>D. melanogaster</i> Model |
| title_fullStr | Antioxidant and Anti-Inflammatory Properties of Quail Yolk Oil via Upregulation of Superoxide Dismutase 1 and Catalase Genes and Downregulation of EIGER and Unpaired 2 Genes in a <i>D. melanogaster</i> Model |
| title_full_unstemmed | Antioxidant and Anti-Inflammatory Properties of Quail Yolk Oil via Upregulation of Superoxide Dismutase 1 and Catalase Genes and Downregulation of EIGER and Unpaired 2 Genes in a <i>D. melanogaster</i> Model |
| title_short | Antioxidant and Anti-Inflammatory Properties of Quail Yolk Oil via Upregulation of Superoxide Dismutase 1 and Catalase Genes and Downregulation of EIGER and Unpaired 2 Genes in a <i>D. melanogaster</i> Model |
| title_sort | antioxidant and anti inflammatory properties of quail yolk oil via upregulation of superoxide dismutase 1 and catalase genes and downregulation of eiger and unpaired 2 genes in a i d melanogaster i model |
| topic | antioxidant effects <i>EIGER</i> and <i>UPD2</i> gene down-regulation fruit fly FTIR GC–MS quail egg oil extracts |
| url | https://www.mdpi.com/2076-3921/13/1/75 |
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