Biological Efficacy of Compounds from Stingless Honey and Sting Honey against Two Pathogenic Bacteria: An In Vitro and In Silico Study
Honey inhibits bacterial growth due to the high sugar concentration, hydrogen peroxide generation, and proteinaceous compounds present in it. In this study, the antibacterial activity of stingless and sting honey against foodborne pathogenic bacteria isolated from spoiled milk samples was examined....
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/19/6536 |
| _version_ | 1797477862315393024 |
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| author | Shirmin Islam Mohammad Joy Pramanik Suvro Biswas Mohammad Moniruzzaman Jui Biswas Mohammad Akhtar-E-Ekram Shahriar Zaman Mohammad Salah Uddin Mohammad Abu Saleh Sabry Hassan |
| author_facet | Shirmin Islam Mohammad Joy Pramanik Suvro Biswas Mohammad Moniruzzaman Jui Biswas Mohammad Akhtar-E-Ekram Shahriar Zaman Mohammad Salah Uddin Mohammad Abu Saleh Sabry Hassan |
| author_sort | Shirmin Islam |
| collection | DOAJ |
| description | Honey inhibits bacterial growth due to the high sugar concentration, hydrogen peroxide generation, and proteinaceous compounds present in it. In this study, the antibacterial activity of stingless and sting honey against foodborne pathogenic bacteria isolated from spoiled milk samples was examined. The isolated bacterial strains were confirmed as <i>Bacillus cereus</i> and <i>Listeria</i><i>monocytogenes</i> through morphological, biochemical, and 16 s RNA analysis. Physiochemical characterizations of the honey samples revealed that both of the honey samples had an acidic pH, low water content, moderate reducing sugar content, and higher proline content. Through the disc diffusion method, the antibacterial activities of the samples were assayed and better results were observed for the 50 mg/disc honey. Both stingless and sting honey showed the most positive efficacy against <i>Bacillus cereus.</i> Therefore, an in silico study was conducted against this bacterium with some common compounds of honey. From several retrieved constituents of stingless and sting honey, 2,4-dihydroxy-2,5-dimethyl 3(2H)-furan-3-one (furan) and 4H-pyran-4-one,2,3-dihydro of both samples and beta.-D-glucopyranose from the stingless revealed high ligand-protein binding efficiencies for the target protein (6d5z, hemolysin II). The root-mean-square deviation, solvent-accessible surface area, the radius of gyration, root-mean-square fluctuations, and hydrogen bonds were used to ensure the binding stability of the docked complexes in the atomistic simulation and confirmed their stability. The combined effort of wet and dry lab-based work support, to some extent, that the antimicrobial properties of honey have great potential for application in medicine as well as in the food industries. |
| first_indexed | 2024-03-09T21:24:45Z |
| format | Article |
| id | doaj.art-81b3c81db24e44de98259fde8b156aa5 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T21:24:45Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-81b3c81db24e44de98259fde8b156aa52023-11-23T21:13:01ZengMDPI AGMolecules1420-30492022-10-012719653610.3390/molecules27196536Biological Efficacy of Compounds from Stingless Honey and Sting Honey against Two Pathogenic Bacteria: An In Vitro and In Silico StudyShirmin Islam0Mohammad Joy Pramanik1Suvro Biswas2Mohammad Moniruzzaman3Jui Biswas4Mohammad Akhtar-E-Ekram5Shahriar Zaman6Mohammad Salah Uddin7Mohammad Abu Saleh8Sabry Hassan9Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, BangladeshMicrobiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, BangladeshMicrobiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, BangladeshMicrobiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, BangladeshMicrobiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, BangladeshMicrobiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, BangladeshMicrobiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, BangladeshMicrobiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, BangladeshMicrobiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, BangladeshDepartment of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaHoney inhibits bacterial growth due to the high sugar concentration, hydrogen peroxide generation, and proteinaceous compounds present in it. In this study, the antibacterial activity of stingless and sting honey against foodborne pathogenic bacteria isolated from spoiled milk samples was examined. The isolated bacterial strains were confirmed as <i>Bacillus cereus</i> and <i>Listeria</i><i>monocytogenes</i> through morphological, biochemical, and 16 s RNA analysis. Physiochemical characterizations of the honey samples revealed that both of the honey samples had an acidic pH, low water content, moderate reducing sugar content, and higher proline content. Through the disc diffusion method, the antibacterial activities of the samples were assayed and better results were observed for the 50 mg/disc honey. Both stingless and sting honey showed the most positive efficacy against <i>Bacillus cereus.</i> Therefore, an in silico study was conducted against this bacterium with some common compounds of honey. From several retrieved constituents of stingless and sting honey, 2,4-dihydroxy-2,5-dimethyl 3(2H)-furan-3-one (furan) and 4H-pyran-4-one,2,3-dihydro of both samples and beta.-D-glucopyranose from the stingless revealed high ligand-protein binding efficiencies for the target protein (6d5z, hemolysin II). The root-mean-square deviation, solvent-accessible surface area, the radius of gyration, root-mean-square fluctuations, and hydrogen bonds were used to ensure the binding stability of the docked complexes in the atomistic simulation and confirmed their stability. The combined effort of wet and dry lab-based work support, to some extent, that the antimicrobial properties of honey have great potential for application in medicine as well as in the food industries.https://www.mdpi.com/1420-3049/27/19/6536honey<i>Bacillus cereus</i><i>Listeria monocytogenes</i>antibacterial activitymolecular dockingmolecular dynamics simulation |
| spellingShingle | Shirmin Islam Mohammad Joy Pramanik Suvro Biswas Mohammad Moniruzzaman Jui Biswas Mohammad Akhtar-E-Ekram Shahriar Zaman Mohammad Salah Uddin Mohammad Abu Saleh Sabry Hassan Biological Efficacy of Compounds from Stingless Honey and Sting Honey against Two Pathogenic Bacteria: An In Vitro and In Silico Study Molecules honey <i>Bacillus cereus</i> <i>Listeria monocytogenes</i> antibacterial activity molecular docking molecular dynamics simulation |
| title | Biological Efficacy of Compounds from Stingless Honey and Sting Honey against Two Pathogenic Bacteria: An In Vitro and In Silico Study |
| title_full | Biological Efficacy of Compounds from Stingless Honey and Sting Honey against Two Pathogenic Bacteria: An In Vitro and In Silico Study |
| title_fullStr | Biological Efficacy of Compounds from Stingless Honey and Sting Honey against Two Pathogenic Bacteria: An In Vitro and In Silico Study |
| title_full_unstemmed | Biological Efficacy of Compounds from Stingless Honey and Sting Honey against Two Pathogenic Bacteria: An In Vitro and In Silico Study |
| title_short | Biological Efficacy of Compounds from Stingless Honey and Sting Honey against Two Pathogenic Bacteria: An In Vitro and In Silico Study |
| title_sort | biological efficacy of compounds from stingless honey and sting honey against two pathogenic bacteria an in vitro and in silico study |
| topic | honey <i>Bacillus cereus</i> <i>Listeria monocytogenes</i> antibacterial activity molecular docking molecular dynamics simulation |
| url | https://www.mdpi.com/1420-3049/27/19/6536 |
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