Species DNA-based identification for detection of processed meat adulteration: is there a role of human short tandem repeats (STRs)?
Abstract Background Species identification in the food of animal origin is an essential aspect of its control. Food safety and environmental forensic professionals in various countries are becoming increasingly concerned about the number of serious food offences being carried out by organised crimin...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-04-01
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| Series: | Egyptian Journal of Forensic Sciences |
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| Online Access: | http://link.springer.com/article/10.1186/s41935-019-0121-y |
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| author | Ghada Ali Omran Asmaa Osama Tolba Eman Ezz El-Dawela El-Sharkawy Doaa Mohammed Abdel-Aziz Hussein Youssef Ahmed |
| author_facet | Ghada Ali Omran Asmaa Osama Tolba Eman Ezz El-Dawela El-Sharkawy Doaa Mohammed Abdel-Aziz Hussein Youssef Ahmed |
| author_sort | Ghada Ali Omran |
| collection | DOAJ |
| description | Abstract Background Species identification in the food of animal origin is an essential aspect of its control. Food safety and environmental forensic professionals in various countries are becoming increasingly concerned about the number of serious food offences being carried out by organised criminals. Adulteration in food especially meat is relevant for legal, economic, religious and public health reasons. This study aimed to determine potential adulteration and/or contamination with the donkey, chicken or even human tissues or cells in different marketed red meat products. The products tested were the uncooked beef burger, sausage, kofta and luncheon, manually processed or were of different commercial brands with variable prices, through a PCR-based method. A total of 40 different commercial meat product samples were randomly collected from restaurants, butchers, hypermarkets and local shops. The 12S rRNA region within the mitochondrial DNA was amplified with species-specific primers for identification of two suspected animal species (donkey and chicken) and two nuclear DNA STRs (short tandem repeats) loci, TPOX and D18S51 for excluding human origin of adulteration or contamination. Results The total beef samples analysed showed 87.5% adulteration and mislabelling with one or more species. They were mostly mixed with chicken meat or their by-products (72.5%) followed by donkey (12.5%) and lastly human (2.5%) that was detected in a manually prepared kofta sample. Conclusion The used non-human species-specific PCR along with the first reported use of human hypervariable STRs proved valuable and straightforward techniques for species authentication of meat products. |
| first_indexed | 2024-12-13T00:12:11Z |
| format | Article |
| id | doaj.art-81ea0f38d5ad4b04895a9c3a1e08846e |
| institution | Directory Open Access Journal |
| issn | 2090-5939 |
| language | English |
| last_indexed | 2024-12-13T00:12:11Z |
| publishDate | 2019-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Forensic Sciences |
| spelling | doaj.art-81ea0f38d5ad4b04895a9c3a1e08846e2022-12-22T00:05:56ZengSpringerOpenEgyptian Journal of Forensic Sciences2090-59392019-04-01911810.1186/s41935-019-0121-ySpecies DNA-based identification for detection of processed meat adulteration: is there a role of human short tandem repeats (STRs)?Ghada Ali Omran0Asmaa Osama Tolba1Eman Ezz El-Dawela El-Sharkawy2Doaa Mohammed Abdel-Aziz3Hussein Youssef Ahmed4Faculty of Medicine, Forensic Medicine and Clinical Toxicology Department, Assiut UniversityHygiene and Control of Meat, Fish and Their Products and Animal By-Products Unit, Assiut University HospitalsFaculty of Veterinary Medicine, Forensic Medicine and Toxicology Department, Assiut UniversityFaculty of Veterinary Medicine, Department of Food Hygiene, Assiut UniversityFaculty of Veterinary Medicine, Department of Food Hygiene, Assiut UniversityAbstract Background Species identification in the food of animal origin is an essential aspect of its control. Food safety and environmental forensic professionals in various countries are becoming increasingly concerned about the number of serious food offences being carried out by organised criminals. Adulteration in food especially meat is relevant for legal, economic, religious and public health reasons. This study aimed to determine potential adulteration and/or contamination with the donkey, chicken or even human tissues or cells in different marketed red meat products. The products tested were the uncooked beef burger, sausage, kofta and luncheon, manually processed or were of different commercial brands with variable prices, through a PCR-based method. A total of 40 different commercial meat product samples were randomly collected from restaurants, butchers, hypermarkets and local shops. The 12S rRNA region within the mitochondrial DNA was amplified with species-specific primers for identification of two suspected animal species (donkey and chicken) and two nuclear DNA STRs (short tandem repeats) loci, TPOX and D18S51 for excluding human origin of adulteration or contamination. Results The total beef samples analysed showed 87.5% adulteration and mislabelling with one or more species. They were mostly mixed with chicken meat or their by-products (72.5%) followed by donkey (12.5%) and lastly human (2.5%) that was detected in a manually prepared kofta sample. Conclusion The used non-human species-specific PCR along with the first reported use of human hypervariable STRs proved valuable and straightforward techniques for species authentication of meat products.http://link.springer.com/article/10.1186/s41935-019-0121-yForensicSpecies identificationMeat productsAdulterationHuman STRs |
| spellingShingle | Ghada Ali Omran Asmaa Osama Tolba Eman Ezz El-Dawela El-Sharkawy Doaa Mohammed Abdel-Aziz Hussein Youssef Ahmed Species DNA-based identification for detection of processed meat adulteration: is there a role of human short tandem repeats (STRs)? Egyptian Journal of Forensic Sciences Forensic Species identification Meat products Adulteration Human STRs |
| title | Species DNA-based identification for detection of processed meat adulteration: is there a role of human short tandem repeats (STRs)? |
| title_full | Species DNA-based identification for detection of processed meat adulteration: is there a role of human short tandem repeats (STRs)? |
| title_fullStr | Species DNA-based identification for detection of processed meat adulteration: is there a role of human short tandem repeats (STRs)? |
| title_full_unstemmed | Species DNA-based identification for detection of processed meat adulteration: is there a role of human short tandem repeats (STRs)? |
| title_short | Species DNA-based identification for detection of processed meat adulteration: is there a role of human short tandem repeats (STRs)? |
| title_sort | species dna based identification for detection of processed meat adulteration is there a role of human short tandem repeats strs |
| topic | Forensic Species identification Meat products Adulteration Human STRs |
| url | http://link.springer.com/article/10.1186/s41935-019-0121-y |
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