Comparative Analysis of Cell–Cell Contact Abundance in Ovarian Carcinoma Cells Cultured in Two- and Three-Dimensional In Vitro Models
Tumor resistance to therapy is associated with the 3D organization and peculiarities of the tumor microenvironment, of which intercellular adhesion is a key participant. In this work, the abundance of contact proteins was compared in SKOV-3 and SKOV-3.ip human ovarian adenocarcinoma cell lines, cult...
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MDPI AG
2020-12-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/9/12/446 |
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| author | Olga M. Kutova Ludmila M. Sencha Anton D. Pospelov Olga E. Dobrynina Anna A. Brilkina Elena I. Cherkasova Irina V. Balalaeva |
| author_facet | Olga M. Kutova Ludmila M. Sencha Anton D. Pospelov Olga E. Dobrynina Anna A. Brilkina Elena I. Cherkasova Irina V. Balalaeva |
| author_sort | Olga M. Kutova |
| collection | DOAJ |
| description | Tumor resistance to therapy is associated with the 3D organization and peculiarities of the tumor microenvironment, of which intercellular adhesion is a key participant. In this work, the abundance of contact proteins was compared in SKOV-3 and SKOV-3.ip human ovarian adenocarcinoma cell lines, cultivated in monolayers, tumor spheroids and collagen hydrogels. Three-dimensional models were characterized by extremely low expression of basic molecules of adherens junctions E-cadherin and demonstrated a simultaneous decrease in desmosomal protein desmoglein-2, gap junction protein connexin-43 and tight junction proteins occludin and ZO-1. The reduction in the level of contact proteins was most pronounced in collagen hydrogel, accompanied by significantly increased resistance to treatment with doxorubicin and targeted anticancer toxin DARPin-LoPE. Thus, we suggest that 3D models of ovarian cancer, especially matrix-based models, tend to recapitulate tumor microenvironment and treatment responsiveness to a greater extent than monolayer culture, so they can be used as a highly relevant platform for drug efficiency evaluation. |
| first_indexed | 2024-03-10T14:18:31Z |
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| id | doaj.art-820487cace494cfc9ba6bf4d3c7ce23b |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T14:18:31Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-820487cace494cfc9ba6bf4d3c7ce23b2023-11-20T23:34:23ZengMDPI AGBiology2079-77372020-12-0191244610.3390/biology9120446Comparative Analysis of Cell–Cell Contact Abundance in Ovarian Carcinoma Cells Cultured in Two- and Three-Dimensional In Vitro ModelsOlga M. Kutova0Ludmila M. Sencha1Anton D. Pospelov2Olga E. Dobrynina3Anna A. Brilkina4Elena I. Cherkasova5Irina V. Balalaeva6The Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Ave., 603950 Nizhny Novgorod, RussiaThe Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Ave., 603950 Nizhny Novgorod, RussiaThe Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Ave., 603950 Nizhny Novgorod, RussiaThe Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Ave., 603950 Nizhny Novgorod, RussiaThe Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Ave., 603950 Nizhny Novgorod, RussiaThe Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Ave., 603950 Nizhny Novgorod, RussiaThe Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Ave., 603950 Nizhny Novgorod, RussiaTumor resistance to therapy is associated with the 3D organization and peculiarities of the tumor microenvironment, of which intercellular adhesion is a key participant. In this work, the abundance of contact proteins was compared in SKOV-3 and SKOV-3.ip human ovarian adenocarcinoma cell lines, cultivated in monolayers, tumor spheroids and collagen hydrogels. Three-dimensional models were characterized by extremely low expression of basic molecules of adherens junctions E-cadherin and demonstrated a simultaneous decrease in desmosomal protein desmoglein-2, gap junction protein connexin-43 and tight junction proteins occludin and ZO-1. The reduction in the level of contact proteins was most pronounced in collagen hydrogel, accompanied by significantly increased resistance to treatment with doxorubicin and targeted anticancer toxin DARPin-LoPE. Thus, we suggest that 3D models of ovarian cancer, especially matrix-based models, tend to recapitulate tumor microenvironment and treatment responsiveness to a greater extent than monolayer culture, so they can be used as a highly relevant platform for drug efficiency evaluation.https://www.mdpi.com/2079-7737/9/12/446cell–cell contactscancer resistancetumor spheroidscollagen hydrogeladherens junctionsdesmosomes |
| spellingShingle | Olga M. Kutova Ludmila M. Sencha Anton D. Pospelov Olga E. Dobrynina Anna A. Brilkina Elena I. Cherkasova Irina V. Balalaeva Comparative Analysis of Cell–Cell Contact Abundance in Ovarian Carcinoma Cells Cultured in Two- and Three-Dimensional In Vitro Models Biology cell–cell contacts cancer resistance tumor spheroids collagen hydrogel adherens junctions desmosomes |
| title | Comparative Analysis of Cell–Cell Contact Abundance in Ovarian Carcinoma Cells Cultured in Two- and Three-Dimensional In Vitro Models |
| title_full | Comparative Analysis of Cell–Cell Contact Abundance in Ovarian Carcinoma Cells Cultured in Two- and Three-Dimensional In Vitro Models |
| title_fullStr | Comparative Analysis of Cell–Cell Contact Abundance in Ovarian Carcinoma Cells Cultured in Two- and Three-Dimensional In Vitro Models |
| title_full_unstemmed | Comparative Analysis of Cell–Cell Contact Abundance in Ovarian Carcinoma Cells Cultured in Two- and Three-Dimensional In Vitro Models |
| title_short | Comparative Analysis of Cell–Cell Contact Abundance in Ovarian Carcinoma Cells Cultured in Two- and Three-Dimensional In Vitro Models |
| title_sort | comparative analysis of cell cell contact abundance in ovarian carcinoma cells cultured in two and three dimensional in vitro models |
| topic | cell–cell contacts cancer resistance tumor spheroids collagen hydrogel adherens junctions desmosomes |
| url | https://www.mdpi.com/2079-7737/9/12/446 |
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