In vivo fluorescence lifetime imaging of macrophage intracellular metabolism during wound responses in zebrafish

In vivo fluorescence lifetime imaging of macrophage intracellular metabolism during wound responses in zebrafish

The function of macrophages in vitro is linked to their metabolic rewiring. However, macrophage metabolism remains poorly characterized in situ. Here, we used two-photon intensity and lifetime imaging of autofluorescent metabolic coenzymes, nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and...

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Bibliographic Details
Main Authors: Veronika Miskolci, Kelsey E Tweed, Michael R Lasarev, Emily C Britt, Alex J Walsh, Landon J Zimmerman, Courtney E McDougal, Mark R Cronan, Jing Fan, John-Demian Sauer, Melissa C Skala, Anna Huttenlocher
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2022-02-01
Series:eLife
Subjects:
FLIM
macrophages
NAD(P)H
optical redox ratio
immunometabolism
wound healing
Online Access:https://elifesciences.org/articles/66080

Internet

https://elifesciences.org/articles/66080

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