| Summary: | 为提高1,3-二油酸-2-棕榈酸甘油三酯（OPO）的含量，以化学酯交换联合酶法酸解反应制备的OPO为原料，采用单因素实验和正交实验考察干法分提条件（结晶温度、冷却速率、养晶时间）对OPO含量及提升率、sn-2位棕榈酸（PA）占总PA含量及提升率和分提液油得率的影响，确定干法分提的最优工艺条件。结果表明，干法分提的最优工艺条件为结晶温度6 ℃、冷却速率4 ℃/h、养晶时间3 h，在此条件下OPO含量为（38.84±0.39）%，OPO含量提升率为（46.07±147）%，sn-2位PA占总PA含量为（91.08±0.16）%，sn-2位PA占总PA含量提升率为（4761±0.25）%，分提液油得率为（31.26±0.34）%。综上，通过干法分提可以获得OPO纯度更高的甘油三酯。
In order to improve the content of 1,3-dioleoyl-2-palmitoylglycerol (OPO), OPO prepared by chemical transesterification combined with enzymatic acid digestion reaction was used as raw material, the effects of dry fractionation conditions (crystallization temperature, cooling rate and crystal raising time) on the content and enhancement rate of OPO, the content and enhancement rate of sn-2 palmitic acid (PA) in total PA and the liquid oil yield of fraction were investigated by single factor experiment and orthogonal experiment. The results showed that the optimal conditions of the dry fractionation were as follows: crystallization temperature 6 ℃, cooling rate 4 ℃/h and crystal raising time 3 h. Under these conditions, the content and enhancement rate of OPO was （38.84±0.39）% and （46.07±1.47）% respectively, the content and enhancement rate of sn-2 PA in total PA was （91.08±0.16）% and（4761±0.25）% respectively, and the liquid oil yield was （31.26±0.34）%. In conclusion, triglyceride with higher purity of OPO can be obtained by dry fractionation.
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