Reconstitution of a eukaryotic replisome reveals suppression mechanisms that define leading/lagging strand operation

Reconstitution of a eukaryotic replisome reveals suppression mechanisms that define leading/lagging strand operation

We have reconstituted a eukaryotic leading/lagging strand replisome comprising 31 distinct polypeptides. This study identifies a process unprecedented in bacterial replisomes. While bacteria and phage simply recruit polymerases to the fork, we find that suppression mechanisms are used to position th...

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Bibliographic Details
Main Authors: Roxana E Georgescu, Grant D Schauer, Nina Y Yao, Lance D Langston, Olga Yurieva, Dan Zhang, Jeff Finkelstein, Mike E O'Donnell
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2015-04-01
Series:eLife
Subjects:
DNA replication
replication fork
CMG
Pol delta
Pol epsilon
Online Access:https://elifesciences.org/articles/04988

Internet

https://elifesciences.org/articles/04988

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