Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

The CRISPR/Cas9 system is a robust genome editing technology that works in human cells, animals and plants based on the RNA-programmed DNA cleaving activity of the Cas9 enzyme. Building on previous work (Jinek et al., 2013), we show here that new genetic information can be introduced site-specifical...

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Bibliographic Details
Main Authors: Steven Lin, Brett T Staahl, Ravi K Alla, Jennifer A Doudna
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2014-12-01
Series:eLife
Subjects:
CRISPR/Cas9
genome engineering
homologous recombination
cell cycle synchronization
nocodazole
non-homologous end joining
Online Access:https://elifesciences.org/articles/04766

Internet

https://elifesciences.org/articles/04766

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