Spectral imaging and nucleic acid mimics fluorescence in situ hybridization (SI-NAM-FISH) for multiplex detection of clinical pathogens

Spectral imaging and nucleic acid mimics fluorescence in situ hybridization (SI-NAM-FISH) for multiplex detection of clinical pathogens

The application of nucleic acid mimics (NAMs), such as locked nucleic acid (LNA) and 2′-O-methyl-RNA (2’OMe), has improved the performance of fluorescence in situ hybridization (FISH) methods for the detection/location of clinical pathogens since they provide design versatility and thermodynamic con...

Full description

Bibliographic Details
Main Authors: Andreia S. Azevedo, Ricardo M. Fernandes, Ana R. Faria, Oscar F. Silvestre, Jana B. Nieder, Chenguang Lou, Jesper Wengel, Carina Almeida, Nuno F. Azevedo
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-09-01
Series:Frontiers in Microbiology
Subjects:
nucleic acid mimics
fluorescence in situ hybridization
clinical pathogens
bacteria
multiplex detection
epifluorescence microscope
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2022.976639/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2022.976639/full

Similar Items