Paraspeckle protein 1 (PSPC1) is involved in the cisplatin induced DNA damage response--role in G1/S checkpoint.
Paraspeckle protein 1 (PSPC1) was first identified as a structural protein of the subnuclear structure termed paraspeckle. However, the exact physiological functions of PSPC1 are still largely unknown. Previously, using a proteomic approach, we have shown that exposure to cisplatin can induce PSPC1...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4018278?pdf=render |
| _version_ | 1819054657734967296 |
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| author | Xiangjing Gao Liya Kong Xianghong Lu Guanglin Zhang Linfeng Chi Ying Jiang Yihua Wu Chunlan Yan Penelope Duerksen-Hughes Xinqiang Zhu Jun Yang |
| author_facet | Xiangjing Gao Liya Kong Xianghong Lu Guanglin Zhang Linfeng Chi Ying Jiang Yihua Wu Chunlan Yan Penelope Duerksen-Hughes Xinqiang Zhu Jun Yang |
| author_sort | Xiangjing Gao |
| collection | DOAJ |
| description | Paraspeckle protein 1 (PSPC1) was first identified as a structural protein of the subnuclear structure termed paraspeckle. However, the exact physiological functions of PSPC1 are still largely unknown. Previously, using a proteomic approach, we have shown that exposure to cisplatin can induce PSPC1 expression in HeLa cells, indicating the possible involvement for PSPC1 in the DNA damage response (DDR). In the current study, the role of PSPC1 in DDR was examined. First, it was found that cisplatin treatment could indeed induce the expression of PSPC1 protein. Abolishing PSPC1 expression by siRNA significantly inhibited cell growth, caused spontaneous cell death, and increased DNA damage. However, PSPC1 did not co-localize with γH2AX, 53BP1, or Rad51, indicating no direct involvement in DNA repair pathways mediated by these molecules. Interestingly, knockdown of PSPC1 disrupted the normal cell cycle distribution, with more cells entering the G2/M phase. Furthermore, while cisplatin induced G1/S arrest in HeLa cells, knockdown of PSPC1 caused cells to escape the G1/S checkpoint and enter mitosis, and resulted in more cell death. Taken together, these observations indicate a new role for PSPC1 in maintaining genome integrity during the DDR, particularly in the G1/S checkpoint. |
| first_indexed | 2024-12-21T12:55:07Z |
| format | Article |
| id | doaj.art-82aca526c0784383999e12a1bcb3c05a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T12:55:07Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-82aca526c0784383999e12a1bcb3c05a2022-12-21T19:03:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0195e9717410.1371/journal.pone.0097174Paraspeckle protein 1 (PSPC1) is involved in the cisplatin induced DNA damage response--role in G1/S checkpoint.Xiangjing GaoLiya KongXianghong LuGuanglin ZhangLinfeng ChiYing JiangYihua WuChunlan YanPenelope Duerksen-HughesXinqiang ZhuJun YangParaspeckle protein 1 (PSPC1) was first identified as a structural protein of the subnuclear structure termed paraspeckle. However, the exact physiological functions of PSPC1 are still largely unknown. Previously, using a proteomic approach, we have shown that exposure to cisplatin can induce PSPC1 expression in HeLa cells, indicating the possible involvement for PSPC1 in the DNA damage response (DDR). In the current study, the role of PSPC1 in DDR was examined. First, it was found that cisplatin treatment could indeed induce the expression of PSPC1 protein. Abolishing PSPC1 expression by siRNA significantly inhibited cell growth, caused spontaneous cell death, and increased DNA damage. However, PSPC1 did not co-localize with γH2AX, 53BP1, or Rad51, indicating no direct involvement in DNA repair pathways mediated by these molecules. Interestingly, knockdown of PSPC1 disrupted the normal cell cycle distribution, with more cells entering the G2/M phase. Furthermore, while cisplatin induced G1/S arrest in HeLa cells, knockdown of PSPC1 caused cells to escape the G1/S checkpoint and enter mitosis, and resulted in more cell death. Taken together, these observations indicate a new role for PSPC1 in maintaining genome integrity during the DDR, particularly in the G1/S checkpoint.http://europepmc.org/articles/PMC4018278?pdf=render |
| spellingShingle | Xiangjing Gao Liya Kong Xianghong Lu Guanglin Zhang Linfeng Chi Ying Jiang Yihua Wu Chunlan Yan Penelope Duerksen-Hughes Xinqiang Zhu Jun Yang Paraspeckle protein 1 (PSPC1) is involved in the cisplatin induced DNA damage response--role in G1/S checkpoint. PLoS ONE |
| title | Paraspeckle protein 1 (PSPC1) is involved in the cisplatin induced DNA damage response--role in G1/S checkpoint. |
| title_full | Paraspeckle protein 1 (PSPC1) is involved in the cisplatin induced DNA damage response--role in G1/S checkpoint. |
| title_fullStr | Paraspeckle protein 1 (PSPC1) is involved in the cisplatin induced DNA damage response--role in G1/S checkpoint. |
| title_full_unstemmed | Paraspeckle protein 1 (PSPC1) is involved in the cisplatin induced DNA damage response--role in G1/S checkpoint. |
| title_short | Paraspeckle protein 1 (PSPC1) is involved in the cisplatin induced DNA damage response--role in G1/S checkpoint. |
| title_sort | paraspeckle protein 1 pspc1 is involved in the cisplatin induced dna damage response role in g1 s checkpoint |
| url | http://europepmc.org/articles/PMC4018278?pdf=render |
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