DETECTION OF SALMONELLA TYPHIMURIUM BACTERIA ON BAKERY PRODUCTS SAMPLES USING BOILING ISOLATION TECHNIQUE
Detection of Salmonella typhimurium ATCC 14028 bacteria in bakery product samples by real-time PCR using boiling isolation technique. The basis of this research is to have an impact on economic value in carrying out the confirmation test for Salmonella typhimurium ATCC 14028, where testing is carri...
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| Format: | Article |
| Language: | Indonesian |
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Universitas Muhammadiyah Magelang
2022-12-01
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| Series: | Jurnal Farmasi Sains dan Praktis |
| Subjects: | |
| Online Access: | http://journal.unimma.ac.id/index.php/pharmacy/article/view/5550 |
| _version_ | 1797987797312733184 |
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| author | Alfi Sophian Ratna Purwaningsih Muhammad Tri Sutrisno Purwadi Purwadi Arif Wahyudi |
| author_facet | Alfi Sophian Ratna Purwaningsih Muhammad Tri Sutrisno Purwadi Purwadi Arif Wahyudi |
| author_sort | Alfi Sophian |
| collection | DOAJ |
| description |
Detection of Salmonella typhimurium ATCC 14028 bacteria in bakery product samples by real-time PCR using boiling isolation technique. The basis of this research is to have an impact on economic value in carrying out the confirmation test for Salmonella typhimurium ATCC 14028, where testing is carried out conventionally will require large costs, so it is necessary to innovate in terms of modifying the testing phase so that it is more effective and efficient. The purpose of this study was to see whether the boiling isolation technique could be used for the detection test for Salmonella typhimurium ATCC 14028 on bacterial product samples. The sample in this study consisted of 15 types of bacterial product samples spiked with Salmonella typhimurium ATCC 14028 cultures that had been cultured into phase 2 working cultures. The method used in this study was qPCR analysis using the SYBR Green method. The results of real-time PCR analysis obtained Ct values in the range 7.55 - 8.91 with an average of 8.28 and a Tm value in the range 85.50 - 86.20 with an average of 85.77 Based on these data it can be concluded that the detection of Salmonella typhimurium bacteria ATCC 14028 with real-time PCR using boiling isolation technique can be applied for testing on bakery product samples.
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| first_indexed | 2024-04-11T07:53:51Z |
| format | Article |
| id | doaj.art-82c7148bb8644452bba941088048277a |
| institution | Directory Open Access Journal |
| issn | 2549-9068 2579-4558 |
| language | Indonesian |
| last_indexed | 2024-04-11T07:53:51Z |
| publishDate | 2022-12-01 |
| publisher | Universitas Muhammadiyah Magelang |
| record_format | Article |
| series | Jurnal Farmasi Sains dan Praktis |
| spelling | doaj.art-82c7148bb8644452bba941088048277a2022-12-22T04:36:00ZindUniversitas Muhammadiyah MagelangJurnal Farmasi Sains dan Praktis2549-90682579-45582022-12-018310.31603/pharmacy.v8i3.5550DETECTION OF SALMONELLA TYPHIMURIUM BACTERIA ON BAKERY PRODUCTS SAMPLES USING BOILING ISOLATION TECHNIQUEAlfi Sophian0Ratna Purwaningsih1Muhammad Tri Sutrisno2Purwadi Purwadi3Arif Wahyudi4National Center for Drug and Food Testing Development, Food and Drug Supervisory Agency. Percetakan Negara Street No. 23. Central Jakarta, 10560, IndonesiaDirectorate of Processed Food Production Supervision, Food and Drug Supervisory Agency. Percetakan Negara Street No. 23. Central Jakarta, 10560, IndonesiaNational Agency for Drug and Food Control Center in Gorontalo, Central, South Toto Street, Bone Bolango, Gorontalo, 96124, IndonesiaNational Agency for Drug and Food Control Center in Gorontalo, Central, South Toto Street, Bone Bolango, Gorontalo, 96124, Indonesia.National Agency for Drug and Food Control Center in Gorontalo, Central, South Toto Street, Bone Bolango, Gorontalo, 96124, Indonesia. Detection of Salmonella typhimurium ATCC 14028 bacteria in bakery product samples by real-time PCR using boiling isolation technique. The basis of this research is to have an impact on economic value in carrying out the confirmation test for Salmonella typhimurium ATCC 14028, where testing is carried out conventionally will require large costs, so it is necessary to innovate in terms of modifying the testing phase so that it is more effective and efficient. The purpose of this study was to see whether the boiling isolation technique could be used for the detection test for Salmonella typhimurium ATCC 14028 on bacterial product samples. The sample in this study consisted of 15 types of bacterial product samples spiked with Salmonella typhimurium ATCC 14028 cultures that had been cultured into phase 2 working cultures. The method used in this study was qPCR analysis using the SYBR Green method. The results of real-time PCR analysis obtained Ct values in the range 7.55 - 8.91 with an average of 8.28 and a Tm value in the range 85.50 - 86.20 with an average of 85.77 Based on these data it can be concluded that the detection of Salmonella typhimurium bacteria ATCC 14028 with real-time PCR using boiling isolation technique can be applied for testing on bakery product samples. http://journal.unimma.ac.id/index.php/pharmacy/article/view/5550BoilingDNAReal-Time PCRSalmonella typhimurium |
| spellingShingle | Alfi Sophian Ratna Purwaningsih Muhammad Tri Sutrisno Purwadi Purwadi Arif Wahyudi DETECTION OF SALMONELLA TYPHIMURIUM BACTERIA ON BAKERY PRODUCTS SAMPLES USING BOILING ISOLATION TECHNIQUE Jurnal Farmasi Sains dan Praktis Boiling DNA Real-Time PCR Salmonella typhimurium |
| title | DETECTION OF SALMONELLA TYPHIMURIUM BACTERIA ON BAKERY PRODUCTS SAMPLES USING BOILING ISOLATION TECHNIQUE |
| title_full | DETECTION OF SALMONELLA TYPHIMURIUM BACTERIA ON BAKERY PRODUCTS SAMPLES USING BOILING ISOLATION TECHNIQUE |
| title_fullStr | DETECTION OF SALMONELLA TYPHIMURIUM BACTERIA ON BAKERY PRODUCTS SAMPLES USING BOILING ISOLATION TECHNIQUE |
| title_full_unstemmed | DETECTION OF SALMONELLA TYPHIMURIUM BACTERIA ON BAKERY PRODUCTS SAMPLES USING BOILING ISOLATION TECHNIQUE |
| title_short | DETECTION OF SALMONELLA TYPHIMURIUM BACTERIA ON BAKERY PRODUCTS SAMPLES USING BOILING ISOLATION TECHNIQUE |
| title_sort | detection of salmonella typhimurium bacteria on bakery products samples using boiling isolation technique |
| topic | Boiling DNA Real-Time PCR Salmonella typhimurium |
| url | http://journal.unimma.ac.id/index.php/pharmacy/article/view/5550 |
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