Voltammetric determination of polyphenolic content in pomegranate juice using a poly(gallic acid)/multiwalled carbon nanotube modified electrode
A simple and sensitive poly(gallic acid)/multiwalled carbon nanotube modified glassy carbon electrode (PGA/MWCNT/GCE) electrochemical sensor was prepared for direct determination of the total phenolic content (TPC) as gallic acid equivalent. The GCE working electrode was electrochemically modified a...
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| Format: | Article |
| Language: | English |
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Beilstein-Institut
2016-07-01
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| Series: | Beilstein Journal of Nanotechnology |
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| Online Access: | https://doi.org/10.3762/bjnano.7.103 |
| _version_ | 1818668838451937280 |
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| author | Refat Abdel-Hamid Emad F. Newair |
| author_facet | Refat Abdel-Hamid Emad F. Newair |
| author_sort | Refat Abdel-Hamid |
| collection | DOAJ |
| description | A simple and sensitive poly(gallic acid)/multiwalled carbon nanotube modified glassy carbon electrode (PGA/MWCNT/GCE) electrochemical sensor was prepared for direct determination of the total phenolic content (TPC) as gallic acid equivalent. The GCE working electrode was electrochemically modified and characterized using scanning electron microscope (SEM), cyclic voltammetry (CV), chronoamperometry and chronocoulometry. It was found that gallic acid (GA) exhibits a superior electrochemical response on the PGA/MWCNT/GCE sensor in comparison with bare GCE. The results reveal that a PGA/MWCNT/GCE sensor can remarkably enhance the electro-oxidation signal of GA as well as shift the peak potentials towards less positive potential values. The dependence of peak current on accumulation potential, accumulation time and pH were investigated by square-wave voltammetry (SWV) to optimize the experimental conditions for the determination of GA. Using the optimized conditions, the sensor responded linearly to a GA concentration throughout the range of 4.97 × 10−6 to 3.38 × 10−5 M with a detection limit of 3.22 × 10−6 M (S/N = 3). The fabricated sensor shows good selectivity, stability, repeatability and (101%) recovery. The sensor was successfully utilized for the determination of total phenolic content in fresh pomegranate juice without interference of ascorbic acid, fructose, potassium nitrate and barbituric acid. The obtained data were compared with the standard Folin–Ciocalteu spectrophotometric results. |
| first_indexed | 2024-12-17T06:42:41Z |
| format | Article |
| id | doaj.art-82e6dd786cd240caaa6354383f193fba |
| institution | Directory Open Access Journal |
| issn | 2190-4286 |
| language | English |
| last_indexed | 2024-12-17T06:42:41Z |
| publishDate | 2016-07-01 |
| publisher | Beilstein-Institut |
| record_format | Article |
| series | Beilstein Journal of Nanotechnology |
| spelling | doaj.art-82e6dd786cd240caaa6354383f193fba2022-12-21T21:59:49ZengBeilstein-InstitutBeilstein Journal of Nanotechnology2190-42862016-07-01711104111210.3762/bjnano.7.1032190-4286-7-103Voltammetric determination of polyphenolic content in pomegranate juice using a poly(gallic acid)/multiwalled carbon nanotube modified electrodeRefat Abdel-Hamid0Emad F. Newair1Unit of Electrochemistry Applications (UEA), Chemistry Department, Faculty of Science, Sohag University, Sohag 82524, EgyptUnit of Electrochemistry Applications (UEA), Chemistry Department, Faculty of Science, Sohag University, Sohag 82524, EgyptA simple and sensitive poly(gallic acid)/multiwalled carbon nanotube modified glassy carbon electrode (PGA/MWCNT/GCE) electrochemical sensor was prepared for direct determination of the total phenolic content (TPC) as gallic acid equivalent. The GCE working electrode was electrochemically modified and characterized using scanning electron microscope (SEM), cyclic voltammetry (CV), chronoamperometry and chronocoulometry. It was found that gallic acid (GA) exhibits a superior electrochemical response on the PGA/MWCNT/GCE sensor in comparison with bare GCE. The results reveal that a PGA/MWCNT/GCE sensor can remarkably enhance the electro-oxidation signal of GA as well as shift the peak potentials towards less positive potential values. The dependence of peak current on accumulation potential, accumulation time and pH were investigated by square-wave voltammetry (SWV) to optimize the experimental conditions for the determination of GA. Using the optimized conditions, the sensor responded linearly to a GA concentration throughout the range of 4.97 × 10−6 to 3.38 × 10−5 M with a detection limit of 3.22 × 10−6 M (S/N = 3). The fabricated sensor shows good selectivity, stability, repeatability and (101%) recovery. The sensor was successfully utilized for the determination of total phenolic content in fresh pomegranate juice without interference of ascorbic acid, fructose, potassium nitrate and barbituric acid. The obtained data were compared with the standard Folin–Ciocalteu spectrophotometric results.https://doi.org/10.3762/bjnano.7.103electrochemical sensorgallic acidmultiwalled carbon nanotubespomegranate juicetotal phenolic content |
| spellingShingle | Refat Abdel-Hamid Emad F. Newair Voltammetric determination of polyphenolic content in pomegranate juice using a poly(gallic acid)/multiwalled carbon nanotube modified electrode Beilstein Journal of Nanotechnology electrochemical sensor gallic acid multiwalled carbon nanotubes pomegranate juice total phenolic content |
| title | Voltammetric determination of polyphenolic content in pomegranate juice using a poly(gallic acid)/multiwalled carbon nanotube modified electrode |
| title_full | Voltammetric determination of polyphenolic content in pomegranate juice using a poly(gallic acid)/multiwalled carbon nanotube modified electrode |
| title_fullStr | Voltammetric determination of polyphenolic content in pomegranate juice using a poly(gallic acid)/multiwalled carbon nanotube modified electrode |
| title_full_unstemmed | Voltammetric determination of polyphenolic content in pomegranate juice using a poly(gallic acid)/multiwalled carbon nanotube modified electrode |
| title_short | Voltammetric determination of polyphenolic content in pomegranate juice using a poly(gallic acid)/multiwalled carbon nanotube modified electrode |
| title_sort | voltammetric determination of polyphenolic content in pomegranate juice using a poly gallic acid multiwalled carbon nanotube modified electrode |
| topic | electrochemical sensor gallic acid multiwalled carbon nanotubes pomegranate juice total phenolic content |
| url | https://doi.org/10.3762/bjnano.7.103 |
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