Development and Clinical Applications of a 5-Plex Real-Time RT-PCR for Swine Enteric Coronaviruses

Development and Clinical Applications of a 5-Plex Real-Time RT-PCR for Swine Enteric Coronaviruses

A PEDV/PDCoV/TGEV/SADS-CoV/XIPC 5-plex real-time RT-PCR was developed and validated for the simultaneous detection and differentiation of four swine enteric coronaviruses (PEDV, PDCoV, TGEV and SADS-CoV) in one PCR reaction (XIPC serves as an exogenous internal positive control). The 5-plex PCR had...

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Bibliographic Details
Main Authors: Jin-Hui Zhu, Gaurav Rawal, Ethan Aljets, Wannarat Yim-Im, Yong-Le Yang, Yao-Wei Huang, Karen Krueger, Phillip Gauger, Rodger Main, Jianqiang Zhang
Format: Article
Language:English
Published: MDPI AG 2022-07-01
Series:Viruses
Subjects:
swine enteric coronavirus
PEDV
PDCoV
TGEV
SADS-CoV
5-plex real-time RT-PCR
Online Access:https://www.mdpi.com/1999-4915/14/7/1536

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https://www.mdpi.com/1999-4915/14/7/1536

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