Detergent-Assisted Protein Digestion—On the Way to Avoid the Key Bottleneck of Shotgun Bottom-Up Proteomics

Detergent-Assisted Protein Digestion—On the Way to Avoid the Key Bottleneck of Shotgun Bottom-Up Proteomics

Gel-free bottom-up shotgun proteomics is the principal methodological platform for the state-of-the-art proteome research. This methodology assumes quantitative isolation of the total protein fraction from a complex biological sample, its limited proteolysis with site-specific proteases, analysis of...

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Bibliographic Details
Main Authors: Katerina Danko, Elena Lukasheva, Vladimir A. Zhukov, Viktor Zgoda, Andrej Frolov
Format: Article
Language:English
Published: MDPI AG 2022-11-01
Series:International Journal of Molecular Sciences
Subjects:
bottom-up proteomics
detergents
detergent-assisted proteolysis
filter-assisted sample preparation (FASP)
gel-free proteomics
shotgun proteomics
Online Access:https://www.mdpi.com/1422-0067/23/22/13903

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https://www.mdpi.com/1422-0067/23/22/13903

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