Identification and validation of reference genes for normalization of gene expression analysis using qRT-PCR in Megalurothrips usitatus (thysanoptera: thripidae)

Identification and validation of reference genes for normalization of gene expression analysis using qRT-PCR in Megalurothrips usitatus (thysanoptera: thripidae)

Introduction: Gene expression analysis by reverse transcription quantitative polymerase chain reaction (qRT-PCR) has been widely used in research including insects. The selection of appropriate reference genes is the key to obtaining accurate and reliable results from qRT-PCR. However, studies on th...

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Bibliographic Details
Main Authors: Qingfang Hou, Linlin Yuan, Haifeng Jin, Han Yan, Fen Li, Shaoying Wu
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-04-01
Series:Frontiers in Physiology
Subjects:
Megalurothrips usitatus
light
qRT-PCR
reference genes
stability
Online Access:https://www.frontiersin.org/articles/10.3389/fphys.2023.1161680/full

Internet

https://www.frontiersin.org/articles/10.3389/fphys.2023.1161680/full

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