Deficiency in FTSJ1 Affects Neuronal Plasticity in the Hippocampal Formation of Mice
The role of the tRNA methyltransferase FTSJ1 in the brain is largely unknown. We analyzed whether FTSJ1-deficient mice (KO) displayed altered neuronal plasticity. We explored open field behavior (10 KO mice (aged 22–25 weeks)) and 11 age-matched control littermates (WT) and examined mean layer thick...
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MDPI AG
2022-07-01
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| author | Viola von Bohlen und Halbach Simone Venz Simon Nwakor Christian Hentschker Elke Hammer Heike Junker Andreas W. Kuss Oliver von Bohlen und Halbach Lars R. Jensen |
| author_facet | Viola von Bohlen und Halbach Simone Venz Simon Nwakor Christian Hentschker Elke Hammer Heike Junker Andreas W. Kuss Oliver von Bohlen und Halbach Lars R. Jensen |
| author_sort | Viola von Bohlen und Halbach |
| collection | DOAJ |
| description | The role of the tRNA methyltransferase FTSJ1 in the brain is largely unknown. We analyzed whether FTSJ1-deficient mice (KO) displayed altered neuronal plasticity. We explored open field behavior (10 KO mice (aged 22–25 weeks)) and 11 age-matched control littermates (WT) and examined mean layer thickness (7 KO; 6 WT) and dendritic spines (5 KO; 5 WT) in the hippocampal area CA1 and the dentate gyrus. Furthermore, long-term potentiation (LTP) within area CA1 was investigated (5 KO; 5 WT), and mass spectrometry (MS) using CA1 tissue (2 each) was performed. Compared to controls, KO mice showed a significant reduction in the mean thickness of apical CA1 layers. Dendritic spine densities were also altered in KO mice. Stable LTP could be induced in the CA1 area of KO mice and remained stable at for at least 1 h, although at a lower level as compared to WTs, while MS data indicated differential abundance of several proteins, which play a role in neuronal plasticity. FTSJ1 has an impact on neuronal plasticity in the murine hippocampal area CA1 at the morphological and physiological levels, which, in conjunction with comparable changes in other cortical areas, might accumulate in disturbed learning and memory functions. |
| first_indexed | 2024-03-09T12:13:57Z |
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| id | doaj.art-8303b52389214846b96c9a4e288a063f |
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| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T12:13:57Z |
| publishDate | 2022-07-01 |
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| series | Biology |
| spelling | doaj.art-8303b52389214846b96c9a4e288a063f2023-11-30T22:49:07ZengMDPI AGBiology2079-77372022-07-01117101110.3390/biology11071011Deficiency in FTSJ1 Affects Neuronal Plasticity in the Hippocampal Formation of MiceViola von Bohlen und Halbach0Simone Venz1Simon Nwakor2Christian Hentschker3Elke Hammer4Heike Junker5Andreas W. Kuss6Oliver von Bohlen und Halbach7Lars R. Jensen8Institute of Anatomy and Cell Biology, University Medicine Greifswald, 17489 Greifswald, GermanyDepartment of Medical Biochemistry and Molecular Biology, University Medicine Greifswald, 17475 Greifswald, GermanyInstitute of Anatomy and Cell Biology, University Medicine Greifswald, 17489 Greifswald, GermanyDepartment of Medical Biochemistry and Molecular Biology, University Medicine Greifswald, 17475 Greifswald, GermanyInterfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, 17475 Greifswald, GermanyDepartment of Medical Biochemistry and Molecular Biology, University Medicine Greifswald, 17475 Greifswald, GermanyInterfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, 17475 Greifswald, GermanyInstitute of Anatomy and Cell Biology, University Medicine Greifswald, 17489 Greifswald, GermanyInterfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, 17475 Greifswald, GermanyThe role of the tRNA methyltransferase FTSJ1 in the brain is largely unknown. We analyzed whether FTSJ1-deficient mice (KO) displayed altered neuronal plasticity. We explored open field behavior (10 KO mice (aged 22–25 weeks)) and 11 age-matched control littermates (WT) and examined mean layer thickness (7 KO; 6 WT) and dendritic spines (5 KO; 5 WT) in the hippocampal area CA1 and the dentate gyrus. Furthermore, long-term potentiation (LTP) within area CA1 was investigated (5 KO; 5 WT), and mass spectrometry (MS) using CA1 tissue (2 each) was performed. Compared to controls, KO mice showed a significant reduction in the mean thickness of apical CA1 layers. Dendritic spine densities were also altered in KO mice. Stable LTP could be induced in the CA1 area of KO mice and remained stable at for at least 1 h, although at a lower level as compared to WTs, while MS data indicated differential abundance of several proteins, which play a role in neuronal plasticity. FTSJ1 has an impact on neuronal plasticity in the murine hippocampal area CA1 at the morphological and physiological levels, which, in conjunction with comparable changes in other cortical areas, might accumulate in disturbed learning and memory functions.https://www.mdpi.com/2079-7737/11/7/1011FTSJ1long-term potentiationtRNA methyl transferaseneuronal plasticityproteomics |
| spellingShingle | Viola von Bohlen und Halbach Simone Venz Simon Nwakor Christian Hentschker Elke Hammer Heike Junker Andreas W. Kuss Oliver von Bohlen und Halbach Lars R. Jensen Deficiency in FTSJ1 Affects Neuronal Plasticity in the Hippocampal Formation of Mice Biology FTSJ1 long-term potentiation tRNA methyl transferase neuronal plasticity proteomics |
| title | Deficiency in FTSJ1 Affects Neuronal Plasticity in the Hippocampal Formation of Mice |
| title_full | Deficiency in FTSJ1 Affects Neuronal Plasticity in the Hippocampal Formation of Mice |
| title_fullStr | Deficiency in FTSJ1 Affects Neuronal Plasticity in the Hippocampal Formation of Mice |
| title_full_unstemmed | Deficiency in FTSJ1 Affects Neuronal Plasticity in the Hippocampal Formation of Mice |
| title_short | Deficiency in FTSJ1 Affects Neuronal Plasticity in the Hippocampal Formation of Mice |
| title_sort | deficiency in ftsj1 affects neuronal plasticity in the hippocampal formation of mice |
| topic | FTSJ1 long-term potentiation tRNA methyl transferase neuronal plasticity proteomics |
| url | https://www.mdpi.com/2079-7737/11/7/1011 |
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