Probing the enzymatic activity and maturation process of the EcAIII Ntn-amidohydrolase using local random mutagenesis

Probing the enzymatic activity and maturation process of the EcAIII Ntn-amidohydrolase using local random mutagenesis

This report describes a comprehensive approach to local random mutagenesis of the E. coli Ntn-amidohydrolase EcAIII, and supplements the results published earlier for the randomization series RDM1. Here, random mutagenesis was applied in the center of the EcAIII molecule, i.e., in the region importa...

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Bibliographic Details
Main Authors: Joanna I. Loch, Anna Ściuk, Marta Kilichowska, Izabela Pieróg, Weronika Łukaszczyk, Katarzyna Zimowska, Mariusz Jaskolski
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-01-01
Series:Acta Biochimica Polonica
Subjects:
L-asparaginase
Ntn-hydrolase
random mutagenesis
enzyme engineering
AlphaFold prediction
Online Access:https://www.frontierspartnerships.org/articles/10.3389/abp.2024.12299/full

Internet

https://www.frontierspartnerships.org/articles/10.3389/abp.2024.12299/full

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