Nanogrid single-nucleus RNA sequencing reveals phenotypic diversity in breast cancer
Single cell RNA sequencing is a powerful tool for understanding cellular diversity but is limited by cost, throughput and sample preparation. Here the authors use nanogrid technology with integrated imaging to sequence thousands of cancer nuclei in parallel from fresh or frozen tissue.
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2017-08-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-017-00244-w |
| _version_ | 1818841051767504896 |
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| author | Ruli Gao Charissa Kim Emi Sei Theodoros Foukakis Nicola Crosetto Leong-Keat Chan Maithreyan Srinivasan Hong Zhang Funda Meric-Bernstam Nicholas Navin |
| author_facet | Ruli Gao Charissa Kim Emi Sei Theodoros Foukakis Nicola Crosetto Leong-Keat Chan Maithreyan Srinivasan Hong Zhang Funda Meric-Bernstam Nicholas Navin |
| author_sort | Ruli Gao |
| collection | DOAJ |
| description | Single cell RNA sequencing is a powerful tool for understanding cellular diversity but is limited by cost, throughput and sample preparation. Here the authors use nanogrid technology with integrated imaging to sequence thousands of cancer nuclei in parallel from fresh or frozen tissue. |
| first_indexed | 2024-12-19T04:19:56Z |
| format | Article |
| id | doaj.art-830f1e7e517147f0b71c9f627efb4d1b |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-19T04:19:56Z |
| publishDate | 2017-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-830f1e7e517147f0b71c9f627efb4d1b2022-12-21T20:36:12ZengNature PortfolioNature Communications2041-17232017-08-018111210.1038/s41467-017-00244-wNanogrid single-nucleus RNA sequencing reveals phenotypic diversity in breast cancerRuli Gao0Charissa Kim1Emi Sei2Theodoros Foukakis3Nicola Crosetto4Leong-Keat Chan5Maithreyan Srinivasan6Hong Zhang7Funda Meric-Bernstam8Nicholas Navin9Department of Genetics, UT MD Anderson Cancer CenterDepartment of Genetics, UT MD Anderson Cancer CenterDepartment of Genetics, UT MD Anderson Cancer CenterDepartment of Oncology-Pathology, Karolinska InstitutetDepartment of Medical Biochemistry and Biophysics, Karolinska InstitutetWafergen Biosystems, IncWafergen Biosystems, IncDepartment of Pathology, UT MD Anderson Cancer CenterDepartment of Investigational Cancer Therapeutics, UT MD Anderson Cancer CenterDepartment of Genetics, UT MD Anderson Cancer CenterSingle cell RNA sequencing is a powerful tool for understanding cellular diversity but is limited by cost, throughput and sample preparation. Here the authors use nanogrid technology with integrated imaging to sequence thousands of cancer nuclei in parallel from fresh or frozen tissue.https://doi.org/10.1038/s41467-017-00244-w |
| spellingShingle | Ruli Gao Charissa Kim Emi Sei Theodoros Foukakis Nicola Crosetto Leong-Keat Chan Maithreyan Srinivasan Hong Zhang Funda Meric-Bernstam Nicholas Navin Nanogrid single-nucleus RNA sequencing reveals phenotypic diversity in breast cancer Nature Communications |
| title | Nanogrid single-nucleus RNA sequencing reveals phenotypic diversity in breast cancer |
| title_full | Nanogrid single-nucleus RNA sequencing reveals phenotypic diversity in breast cancer |
| title_fullStr | Nanogrid single-nucleus RNA sequencing reveals phenotypic diversity in breast cancer |
| title_full_unstemmed | Nanogrid single-nucleus RNA sequencing reveals phenotypic diversity in breast cancer |
| title_short | Nanogrid single-nucleus RNA sequencing reveals phenotypic diversity in breast cancer |
| title_sort | nanogrid single nucleus rna sequencing reveals phenotypic diversity in breast cancer |
| url | https://doi.org/10.1038/s41467-017-00244-w |
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