Establishment of an Efficient Genome Editing System in Lettuce Without Sacrificing Specificity

The efficiency of the CRISPR/Cas9 genome editing system remains limited in many crops. Utilizing strong promoters to boost the expression level of Cas9 are commonly used to improve the editing efficiency. However, these strategies also increase the risk of off-target mutation. Here, we developed a n...

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Bibliographic Details
Main Authors: Wenbo Pan, Xue Liu, Dayong Li, Huawei Zhang
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-06-01
Series:Frontiers in Plant Science
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2022.930592/full