A modified CUT&RUN protocol and analysis pipeline to identify transcription factor binding sites in human cell lines

Summary: CUT&RUN is a recently developed in situ chromatin profiling technique that enables high-resolution chromatin mapping and probing. Herein, we describe our adapted CUT&RUN protocol for transcription factors (TFs). Our protocol outlines all necessary steps for TF profiling including th...

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Bibliographic Details
Main Authors: Nikki Ruoxi Kong, Li Chai, Daniel Geoffrey Tenen, Mahmoud Adel Bassal
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721004573