A modified CUT&RUN protocol and analysis pipeline to identify transcription factor binding sites in human cell lines
Summary: CUT&RUN is a recently developed in situ chromatin profiling technique that enables high-resolution chromatin mapping and probing. Herein, we describe our adapted CUT&RUN protocol for transcription factors (TFs). Our protocol outlines all necessary steps for TF profiling including th...
Main Authors: | Nikki Ruoxi Kong, Li Chai, Daniel Geoffrey Tenen, Mahmoud Adel Bassal |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2021-09-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721004573 |
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