The enhancement of bioactive phytochemicals in agarwood leaves by post-harvest application using yeast extract elicitors and evaluation of their bioactivities
ABSTRACTAgarwood (Aquilaria crassna) leaves are commonly used as an herbal tea for health supplements. The present study aims to develop the post-harvest process of agarwood leaves using yeast extract (YE) as an elicitor and evaluate its anti-inflammatory and anti-diabetic effects. The results showe...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/09540105.2023.2265688 |
| _version_ | 1797447858635407360 |
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| author | Rattanathorn Choonong Jakkrit Jabsanthia Varinda Waewaram Khunkhang Butdapheng Boonchoo Sritularak Waraporn Putalun |
| author_facet | Rattanathorn Choonong Jakkrit Jabsanthia Varinda Waewaram Khunkhang Butdapheng Boonchoo Sritularak Waraporn Putalun |
| author_sort | Rattanathorn Choonong |
| collection | DOAJ |
| description | ABSTRACTAgarwood (Aquilaria crassna) leaves are commonly used as an herbal tea for health supplements. The present study aims to develop the post-harvest process of agarwood leaves using yeast extract (YE) as an elicitor and evaluate its anti-inflammatory and anti-diabetic effects. The results showed that 1% of YE elicitation for 120 min significantly increased genkwanin 5-O-β-primevoside (1.8-fold), mangiferin (1.3-fold), and total benzophenones (1.2-fold) contents over the control group. The phenylalanine ammonia-lyase (PAL) activity increased in maximal at 30 min after YE treatment. The agarwood leaf elicitation with 1% YE for 120 min showed a higher anti-inflammation effect by down-regulation of iNOS, IL-6, and COX-2 in LPS-stimulated RAW264.7 cells and an anti-diabetic effect by enhanced AMPK-α1, AMPK-α2, and GLUT4 in L6 cells over the non-treatment. Our results suggest that YE could be a biotic elicitor to enhance the phytochemical contents and increase the benefit of agarwood leaves as a health supplement. |
| first_indexed | 2024-03-09T14:02:02Z |
| format | Article |
| id | doaj.art-83761ad4ef9641a195a1d0c4682cdbbb |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-03-09T14:02:02Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-83761ad4ef9641a195a1d0c4682cdbbb2023-11-30T06:57:34ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432023-12-0134110.1080/09540105.2023.2265688The enhancement of bioactive phytochemicals in agarwood leaves by post-harvest application using yeast extract elicitors and evaluation of their bioactivitiesRattanathorn Choonong0Jakkrit Jabsanthia1Varinda Waewaram2Khunkhang Butdapheng3Boonchoo Sritularak4Waraporn Putalun5Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, ThailandFaculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, ThailandFaculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, ThailandFaculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, ThailandDepartment of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, ThailandFaculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, ThailandABSTRACTAgarwood (Aquilaria crassna) leaves are commonly used as an herbal tea for health supplements. The present study aims to develop the post-harvest process of agarwood leaves using yeast extract (YE) as an elicitor and evaluate its anti-inflammatory and anti-diabetic effects. The results showed that 1% of YE elicitation for 120 min significantly increased genkwanin 5-O-β-primevoside (1.8-fold), mangiferin (1.3-fold), and total benzophenones (1.2-fold) contents over the control group. The phenylalanine ammonia-lyase (PAL) activity increased in maximal at 30 min after YE treatment. The agarwood leaf elicitation with 1% YE for 120 min showed a higher anti-inflammation effect by down-regulation of iNOS, IL-6, and COX-2 in LPS-stimulated RAW264.7 cells and an anti-diabetic effect by enhanced AMPK-α1, AMPK-α2, and GLUT4 in L6 cells over the non-treatment. Our results suggest that YE could be a biotic elicitor to enhance the phytochemical contents and increase the benefit of agarwood leaves as a health supplement.https://www.tandfonline.com/doi/10.1080/09540105.2023.2265688Agarwood leavesAquilaria crassnapost-harvestyeast extractanti-inflammatory activityanti-diabetic activity |
| spellingShingle | Rattanathorn Choonong Jakkrit Jabsanthia Varinda Waewaram Khunkhang Butdapheng Boonchoo Sritularak Waraporn Putalun The enhancement of bioactive phytochemicals in agarwood leaves by post-harvest application using yeast extract elicitors and evaluation of their bioactivities Food and Agricultural Immunology Agarwood leaves Aquilaria crassna post-harvest yeast extract anti-inflammatory activity anti-diabetic activity |
| title | The enhancement of bioactive phytochemicals in agarwood leaves by post-harvest application using yeast extract elicitors and evaluation of their bioactivities |
| title_full | The enhancement of bioactive phytochemicals in agarwood leaves by post-harvest application using yeast extract elicitors and evaluation of their bioactivities |
| title_fullStr | The enhancement of bioactive phytochemicals in agarwood leaves by post-harvest application using yeast extract elicitors and evaluation of their bioactivities |
| title_full_unstemmed | The enhancement of bioactive phytochemicals in agarwood leaves by post-harvest application using yeast extract elicitors and evaluation of their bioactivities |
| title_short | The enhancement of bioactive phytochemicals in agarwood leaves by post-harvest application using yeast extract elicitors and evaluation of their bioactivities |
| title_sort | enhancement of bioactive phytochemicals in agarwood leaves by post harvest application using yeast extract elicitors and evaluation of their bioactivities |
| topic | Agarwood leaves Aquilaria crassna post-harvest yeast extract anti-inflammatory activity anti-diabetic activity |
| url | https://www.tandfonline.com/doi/10.1080/09540105.2023.2265688 |
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