A novel test for type-I allergy based on crosslink formation of immunoglobulin-E receptors by allergen-specific immunoglobulin-E antibodies and an allergen
Abstract Detection of allergen-specific immunoglobulin E (IgE) antibodies (Abs) in serum would allow for screening of the causative allergen in patients with type-I allergy. In this study, we developed a new assay method to detect allergen-specific IgE Abs, which involved crosslinking the plural Fcε...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-46730-8 |
| _version_ | 1797630318302199808 |
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| author | Yuki Koga Soichiro Ishii Tomoharu Yokooji Konomi Yamamoto Ryohei Ogino Takanori Taogoshi Hiroaki Matsuo |
| author_facet | Yuki Koga Soichiro Ishii Tomoharu Yokooji Konomi Yamamoto Ryohei Ogino Takanori Taogoshi Hiroaki Matsuo |
| author_sort | Yuki Koga |
| collection | DOAJ |
| description | Abstract Detection of allergen-specific immunoglobulin E (IgE) antibodies (Abs) in serum would allow for screening of the causative allergen in patients with type-I allergy. In this study, we developed a new assay method to detect allergen-specific IgE Abs, which involved crosslinking the plural FcεRIα molecules with an allergen and detection using an amplified luminescence proximity homogeneous assay (AlphaCL). First, the allergen concentration, bead concentrations, and incubation time were optimized for the detection of anti-2,4-dinitrophenyl (DNP) IgE Abs in buffer. Under optimal conditions, AlphaCL was able to detect DNP-specific IgE Abs in simulated human serum at levels comparable to those in serum from type-I allergic patients. When AlphaCL was used to detect anti-DNP IgE Abs, no signal counts were obtained with the monovalent allergen 2,4-dinitrophenylated poly-γ-glutamic acid, whereas high signal counts were obtained with the multivalent allergen DNP-BSA. This confirmed that AlphaCL could specifically detect allergen-specific IgE Abs with the ability to crosslink a multivalent allergen. In summary, we have established a new assay model using AlphaCL to detect allergen-specific IgE Abs with FcεRIα crosslinking ability in human serum. This simple and practical assay model may be applied as a new diagnostic tool for patients with type-I allergy. |
| first_indexed | 2024-03-11T11:05:29Z |
| format | Article |
| id | doaj.art-8387e5d6a1184073888cf82f311a6804 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-11T11:05:29Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-8387e5d6a1184073888cf82f311a68042023-11-12T12:13:41ZengNature PortfolioScientific Reports2045-23222023-11-011311810.1038/s41598-023-46730-8A novel test for type-I allergy based on crosslink formation of immunoglobulin-E receptors by allergen-specific immunoglobulin-E antibodies and an allergenYuki Koga0Soichiro Ishii1Tomoharu Yokooji2Konomi Yamamoto3Ryohei Ogino4Takanori Taogoshi5Hiroaki Matsuo6Department of Pharmaceutical Services, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityDepartment of Pharmaceutical Services, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityDepartment of Pharmaceutical Services, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityDepartment of Frontier Science for Pharmacotherapy, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityDepartment of Frontier Science for Pharmacotherapy, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityDepartment of Pharmaceutical Services, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityDepartment of Pharmaceutical Services, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityAbstract Detection of allergen-specific immunoglobulin E (IgE) antibodies (Abs) in serum would allow for screening of the causative allergen in patients with type-I allergy. In this study, we developed a new assay method to detect allergen-specific IgE Abs, which involved crosslinking the plural FcεRIα molecules with an allergen and detection using an amplified luminescence proximity homogeneous assay (AlphaCL). First, the allergen concentration, bead concentrations, and incubation time were optimized for the detection of anti-2,4-dinitrophenyl (DNP) IgE Abs in buffer. Under optimal conditions, AlphaCL was able to detect DNP-specific IgE Abs in simulated human serum at levels comparable to those in serum from type-I allergic patients. When AlphaCL was used to detect anti-DNP IgE Abs, no signal counts were obtained with the monovalent allergen 2,4-dinitrophenylated poly-γ-glutamic acid, whereas high signal counts were obtained with the multivalent allergen DNP-BSA. This confirmed that AlphaCL could specifically detect allergen-specific IgE Abs with the ability to crosslink a multivalent allergen. In summary, we have established a new assay model using AlphaCL to detect allergen-specific IgE Abs with FcεRIα crosslinking ability in human serum. This simple and practical assay model may be applied as a new diagnostic tool for patients with type-I allergy.https://doi.org/10.1038/s41598-023-46730-8 |
| spellingShingle | Yuki Koga Soichiro Ishii Tomoharu Yokooji Konomi Yamamoto Ryohei Ogino Takanori Taogoshi Hiroaki Matsuo A novel test for type-I allergy based on crosslink formation of immunoglobulin-E receptors by allergen-specific immunoglobulin-E antibodies and an allergen Scientific Reports |
| title | A novel test for type-I allergy based on crosslink formation of immunoglobulin-E receptors by allergen-specific immunoglobulin-E antibodies and an allergen |
| title_full | A novel test for type-I allergy based on crosslink formation of immunoglobulin-E receptors by allergen-specific immunoglobulin-E antibodies and an allergen |
| title_fullStr | A novel test for type-I allergy based on crosslink formation of immunoglobulin-E receptors by allergen-specific immunoglobulin-E antibodies and an allergen |
| title_full_unstemmed | A novel test for type-I allergy based on crosslink formation of immunoglobulin-E receptors by allergen-specific immunoglobulin-E antibodies and an allergen |
| title_short | A novel test for type-I allergy based on crosslink formation of immunoglobulin-E receptors by allergen-specific immunoglobulin-E antibodies and an allergen |
| title_sort | novel test for type i allergy based on crosslink formation of immunoglobulin e receptors by allergen specific immunoglobulin e antibodies and an allergen |
| url | https://doi.org/10.1038/s41598-023-46730-8 |
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