Spontaneous Attenuation of Alcoholic Fermentation via the Dysfunction of Cyc8p in <i>Saccharomyces cerevisiae</i>
A cell population characterized by the release of glucose repression and known as [<i>GAR<sup>+</sup></i>] emerges spontaneously in the yeast <i>Saccharomyces cerevisiae</i>. This study revealed that the [<i>GAR<sup>+</sup></i>] variants ex...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/1/304 |
| _version_ | 1797358670819885056 |
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| author | Daisuke Watanabe Maika Kumano Yukiko Sugimoto Hiroshi Takagi |
| author_facet | Daisuke Watanabe Maika Kumano Yukiko Sugimoto Hiroshi Takagi |
| author_sort | Daisuke Watanabe |
| collection | DOAJ |
| description | A cell population characterized by the release of glucose repression and known as [<i>GAR<sup>+</sup></i>] emerges spontaneously in the yeast <i>Saccharomyces cerevisiae</i>. This study revealed that the [<i>GAR<sup>+</sup></i>] variants exhibit retarded alcoholic fermentation when glucose is the sole carbon source. To identify the key to the altered glucose response, the gene expression profile of [<i>GAR<sup>+</sup></i>] cells was examined. Based on RNA-seq data, the [<i>GAR<sup>+</sup></i>] status was linked to impaired function of the Cyc8p–Tup1p complex. Loss of Cyc8p led to a decrease in the initial rate of alcoholic fermentation under glucose-rich conditions via the inactivation of pyruvate decarboxylase, an enzyme unique to alcoholic fermentation. These results suggest that Cyc8p can become inactive to attenuate alcoholic fermentation. These findings may contribute to the elucidation of the mechanism of non-genetic heterogeneity in yeast alcoholic fermentation. |
| first_indexed | 2024-03-08T15:05:25Z |
| format | Article |
| id | doaj.art-838a6c1fc5bf417090c2dc355af5bffd |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T15:05:25Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-838a6c1fc5bf417090c2dc355af5bffd2024-01-10T14:58:56ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0125130410.3390/ijms25010304Spontaneous Attenuation of Alcoholic Fermentation via the Dysfunction of Cyc8p in <i>Saccharomyces cerevisiae</i>Daisuke Watanabe0Maika Kumano1Yukiko Sugimoto2Hiroshi Takagi3Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayamacho, Ikoma 630-0192, Nara, JapanDivision of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayamacho, Ikoma 630-0192, Nara, JapanDivision of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayamacho, Ikoma 630-0192, Nara, JapanDivision of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayamacho, Ikoma 630-0192, Nara, JapanA cell population characterized by the release of glucose repression and known as [<i>GAR<sup>+</sup></i>] emerges spontaneously in the yeast <i>Saccharomyces cerevisiae</i>. This study revealed that the [<i>GAR<sup>+</sup></i>] variants exhibit retarded alcoholic fermentation when glucose is the sole carbon source. To identify the key to the altered glucose response, the gene expression profile of [<i>GAR<sup>+</sup></i>] cells was examined. Based on RNA-seq data, the [<i>GAR<sup>+</sup></i>] status was linked to impaired function of the Cyc8p–Tup1p complex. Loss of Cyc8p led to a decrease in the initial rate of alcoholic fermentation under glucose-rich conditions via the inactivation of pyruvate decarboxylase, an enzyme unique to alcoholic fermentation. These results suggest that Cyc8p can become inactive to attenuate alcoholic fermentation. These findings may contribute to the elucidation of the mechanism of non-genetic heterogeneity in yeast alcoholic fermentation.https://www.mdpi.com/1422-0067/25/1/304alcoholic fermentationCyc8p–Tup1p complex[<i>GAR<sup>+</sup></i>]glucose repressionnon-genetic heterogeneitypyruvate decarboxylase |
| spellingShingle | Daisuke Watanabe Maika Kumano Yukiko Sugimoto Hiroshi Takagi Spontaneous Attenuation of Alcoholic Fermentation via the Dysfunction of Cyc8p in <i>Saccharomyces cerevisiae</i> International Journal of Molecular Sciences alcoholic fermentation Cyc8p–Tup1p complex [<i>GAR<sup>+</sup></i>] glucose repression non-genetic heterogeneity pyruvate decarboxylase |
| title | Spontaneous Attenuation of Alcoholic Fermentation via the Dysfunction of Cyc8p in <i>Saccharomyces cerevisiae</i> |
| title_full | Spontaneous Attenuation of Alcoholic Fermentation via the Dysfunction of Cyc8p in <i>Saccharomyces cerevisiae</i> |
| title_fullStr | Spontaneous Attenuation of Alcoholic Fermentation via the Dysfunction of Cyc8p in <i>Saccharomyces cerevisiae</i> |
| title_full_unstemmed | Spontaneous Attenuation of Alcoholic Fermentation via the Dysfunction of Cyc8p in <i>Saccharomyces cerevisiae</i> |
| title_short | Spontaneous Attenuation of Alcoholic Fermentation via the Dysfunction of Cyc8p in <i>Saccharomyces cerevisiae</i> |
| title_sort | spontaneous attenuation of alcoholic fermentation via the dysfunction of cyc8p in i saccharomyces cerevisiae i |
| topic | alcoholic fermentation Cyc8p–Tup1p complex [<i>GAR<sup>+</sup></i>] glucose repression non-genetic heterogeneity pyruvate decarboxylase |
| url | https://www.mdpi.com/1422-0067/25/1/304 |
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