Development and Evaluation of a Novel One-Step RT-qPCR Targeting the Vero Gene for the Identification of False-Positive Results Caused by Inactivated Virus Vaccine Contamination

To identify false-positive SARS-CoV-2 test results caused by novel coronavirus inactivated vaccine contamination, a novel RT-qPCR targeting the ORF1ab and N genes of SARS-CoV-2 and Vero gene was developed. The amplification efficiency, precision, and lower limit of detection (LLOD) of the RT-qPCR as...

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Bibliographic Details
Main Authors: Xin-Qi Zheng, Wan-Bao Yang, Lin Xie, Zi-Han Wei, Jiang-Xing Zhuang, Tian-Ci Yang
Format: Article
Language:English
Published: MDPI AG 2023-02-01
Series:Vaccines
Subjects:
Online Access:https://www.mdpi.com/2076-393X/11/2/372