Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection

Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection

Genome editing in induced pluripotent stem cells is currently hampered by the laborious and expensive nature of identifying homology-directed repair (HDR)-modified cells. We present an approach where isolation of cells bearing a selectable, HDR-mediated editing event at one locus enriches for HDR-me...

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Bibliographic Details
Main Authors: Katie A. Mitzelfelt, Chris McDermott-Roe, Michael N. Grzybowski, Maribel Marquez, Chieh-Ti Kuo, Michael Riedel, Shuping Lai, Melinda J. Choi, Kurt D. Kolander, Daniel Helbling, David P. Dimmock, Michele A. Battle, Chuanchau J. Jou, Martin Tristani-Firouzi, James W. Verbsky, Ivor J. Benjamin, Aron M. Geurts
Format: Article
Language:English
Published: Elsevier 2017-03-01
Series:Stem Cell Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2213671117300371

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http://www.sciencedirect.com/science/article/pii/S2213671117300371

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