Genome-Scale Identification of Resistance Functions in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Using Tn-seq
ABSTRACT We describe a deep-sequencing procedure for tracking large numbers of transposon mutants of Pseudomonas aeruginosa. The procedure employs a new Tn-seq methodology based on the generation and amplification of single-strand circles carrying transposon junction sequences (the Tn-seq circle met...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
American Society for Microbiology
2011-03-01
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Series: | mBio |
Online Access: | https://journals.asm.org/doi/10.1128/mBio.00315-10 |