Genome-Scale Identification of Resistance Functions in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Using Tn-seq

ABSTRACT We describe a deep-sequencing procedure for tracking large numbers of transposon mutants of Pseudomonas aeruginosa. The procedure employs a new Tn-seq methodology based on the generation and amplification of single-strand circles carrying transposon junction sequences (the Tn-seq circle met...

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Bibliographic Details
Main Authors: Larry A. Gallagher, Jay Shendure, Colin Manoil
Format: Article
Language:English
Published: American Society for Microbiology 2011-03-01
Series:mBio
Online Access:https://journals.asm.org/doi/10.1128/mBio.00315-10