Development of an immunoFET biosensor for the detection of biotinylated PCR product

ImmunoFET (IMFET) biosensor is a simple platform for the detection of biotinylated products of polymerase chain reaction (PCR). Construction of the IMFET biosensor started with adsorption of 1.5 mg/mL of protein A (PA) onto the insulated gate surface of ISFET for 90 min. Next, the immobilized 1/500...

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Bibliographic Details
Main Authors: Wannaporn Muangsuwan, Chamras Promptmas, Wutthinan Jeamsaksiri, Win Bunjongpru, Awirut Srisuwan, Charndet Hruanun, Amporn Poyai, Prapimpun Wongchitrat, Montri Yasawong
Format: Article
Language:English
Published: Elsevier 2016-10-01
Series:Heliyon
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2405844016304789