Genetic diversity of Lactobacillus spp of natural Ethawah goat milk fermented was determined by using 16srDNA with DDGE analysis
The aim of this research is to characterize of Lactobacillus spp. from natural Ethawah goat milk-fermented were using 16SrDNA by denaturation gradient gel electrophoresis (DGGE) analysis. Goat and bovine (as negative control) milk naturally fermented among 24hour until 6 days. Morphological and bioc...
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| Format: | Article |
| Language: | English |
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Indonesian Biological Society
2014-02-01
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| Series: | Journal of Biological Researches |
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| Online Access: | http://berkalahayati.org/journal/afe4567e |
| _version_ | 1818427044479893504 |
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| author | Nur Kusmiyati Nur Hidayat Fatchiyah Fatchiyah |
| author_facet | Nur Kusmiyati Nur Hidayat Fatchiyah Fatchiyah |
| author_sort | Nur Kusmiyati |
| collection | DOAJ |
| description | The aim of this research is to characterize of Lactobacillus spp. from natural Ethawah goat milk-fermented were using 16SrDNA by denaturation gradient gel electrophoresis (DGGE) analysis. Goat and bovine (as negative control) milk naturally fermented among 24hour until 6 days. Morphological and biochemistry of bacteria were characterized by standard methods. The total DNA of bacteria were isolated using alkali lysis, PCR amplification was carried out using 3 pairs of specific primers, DNA-amplified using DGGE and then to determine the relationship among Lactobacteria using NTsys package software V2.0. Phenotypical and biochemical study showed that the 11 strains are belonging to genus Lactobacillus. The dendogram results show all of isolates had similarity characters with genus Lactobacillus around 56-76%. According to morphological and DDGE profiles, we were identified that bacteria isolate of goat milk-fermented are K1A, and K3A are L. casei,and bacteria
isolate K3B is L. plantarum. |
| first_indexed | 2024-12-14T14:39:28Z |
| format | Article |
| id | doaj.art-83e4c3fb919941008dd46b9a2cc4a7db |
| institution | Directory Open Access Journal |
| issn | 0852-6834 2337-389X |
| language | English |
| last_indexed | 2024-12-14T14:39:28Z |
| publishDate | 2014-02-01 |
| publisher | Indonesian Biological Society |
| record_format | Article |
| series | Journal of Biological Researches |
| spelling | doaj.art-83e4c3fb919941008dd46b9a2cc4a7db2022-12-21T22:57:28ZengIndonesian Biological SocietyJournal of Biological Researches0852-68342337-389X2014-02-01182919410.23869/bphjbr.18.2.20133Genetic diversity of Lactobacillus spp of natural Ethawah goat milk fermented was determined by using 16srDNA with DDGE analysisNur Kusmiyati0Nur Hidayat1Fatchiyah Fatchiyah2Biology Departement, Faculty of Mathematic and Natural Science, Brawijaya UniversityFaculty of Food Science & Technology, Brawijaya UniversityBiology Departement, Faculty of Mathematic and Natural Science, Brawijaya UniversityThe aim of this research is to characterize of Lactobacillus spp. from natural Ethawah goat milk-fermented were using 16SrDNA by denaturation gradient gel electrophoresis (DGGE) analysis. Goat and bovine (as negative control) milk naturally fermented among 24hour until 6 days. Morphological and biochemistry of bacteria were characterized by standard methods. The total DNA of bacteria were isolated using alkali lysis, PCR amplification was carried out using 3 pairs of specific primers, DNA-amplified using DGGE and then to determine the relationship among Lactobacteria using NTsys package software V2.0. Phenotypical and biochemical study showed that the 11 strains are belonging to genus Lactobacillus. The dendogram results show all of isolates had similarity characters with genus Lactobacillus around 56-76%. According to morphological and DDGE profiles, we were identified that bacteria isolate of goat milk-fermented are K1A, and K3A are L. casei,and bacteria isolate K3B is L. plantarum.http://berkalahayati.org/journal/afe4567eEthawah goatLactobacillusnatural milk-fermented |
| spellingShingle | Nur Kusmiyati Nur Hidayat Fatchiyah Fatchiyah Genetic diversity of Lactobacillus spp of natural Ethawah goat milk fermented was determined by using 16srDNA with DDGE analysis Journal of Biological Researches Ethawah goat Lactobacillus natural milk-fermented |
| title | Genetic diversity of Lactobacillus spp of natural Ethawah goat milk fermented was determined by using 16srDNA with DDGE analysis |
| title_full | Genetic diversity of Lactobacillus spp of natural Ethawah goat milk fermented was determined by using 16srDNA with DDGE analysis |
| title_fullStr | Genetic diversity of Lactobacillus spp of natural Ethawah goat milk fermented was determined by using 16srDNA with DDGE analysis |
| title_full_unstemmed | Genetic diversity of Lactobacillus spp of natural Ethawah goat milk fermented was determined by using 16srDNA with DDGE analysis |
| title_short | Genetic diversity of Lactobacillus spp of natural Ethawah goat milk fermented was determined by using 16srDNA with DDGE analysis |
| title_sort | genetic diversity of lactobacillus spp of natural ethawah goat milk fermented was determined by using 16srdna with ddge analysis |
| topic | Ethawah goat Lactobacillus natural milk-fermented |
| url | http://berkalahayati.org/journal/afe4567e |
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