Identification of Pri-miRNA Stem-Loop Interacting Proteins in Plants Using a Modified Version of the Csy4 CRISPR Endonuclease

Identification of Pri-miRNA Stem-Loop Interacting Proteins in Plants Using a Modified Version of the Csy4 CRISPR Endonuclease

Regulation at the RNA level by RNA-binding proteins (RBPs) and microRNAs (miRNAs) is key to coordinating eukaryotic gene expression. In plants, the importance of miRNAs is highlighted by severe developmental defects in mutants impaired in miRNA biogenesis. MiRNAs are processed from long primary-micr...

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Bibliographic Details
Main Authors: Janina Lüders, Andreas R. Winkel, Marlene Reichel, Valentin W. Bitterer, Marion Scheibe, Christiane Widmann, Falk Butter, Tino Köster
Format: Article
Language:English
Published: MDPI AG 2022-08-01
Series:International Journal of Molecular Sciences
Subjects:
RNA-binding protein
pri-miRNA stem-loop
RNA-protein interaction
RNA pulldown
Csy4* CRISPR endonuclease
Arabidopsis
Online Access:https://www.mdpi.com/1422-0067/23/16/8961

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https://www.mdpi.com/1422-0067/23/16/8961

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