A quadruplex real-time PCR assay combined with a conventional PCR for the differential detection of Marek’s disease virus vaccines and field strains

A quadruplex real-time PCR assay combined with a conventional PCR for the differential detection of Marek’s disease virus vaccines and field strains

To evaluate the effect of the vaccine and differentiate vaccine from virulent MDV, a new quadruplex real-time PCR assay based on TaqMan probes was developed to differentiate and accurately quantify HVT, CVI988 and virulent MDV-1. The results showed that the limit of detection (LOD) of the new assay...

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Bibliographic Details
Main Authors: Shaopeng Wu, Tian Ding, Hongxia Shao, Kun Qian, Jianqiang Ye, Aijian Qin
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-05-01
Series:Frontiers in Veterinary Science
Subjects:
Marek’s disease virus
vaccination
CVI988/Rispens
real-time PCR
detection
Online Access:https://www.frontiersin.org/articles/10.3389/fvets.2023.1161441/full

Internet

https://www.frontiersin.org/articles/10.3389/fvets.2023.1161441/full

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