Labeling of high density lipoproteins with [3H] acetic anhydride

Rat serum HDL was labeled by reaction with [3H] acetic anhydride at pH 7.2 for 30 min at room temperature by a modification of the method of Montelaro and Rueckert (1975. J. Biol. Chem. 250: 1413). Protein specific activities of 60 dpm/ng were achieved. Seven percent of the label was in lipid, of wh...

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Bibliographic Details
Main Author: J B Marsh
Format: Article
Language:English
Published: Elsevier 1978-01-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520415822