Co-Transfected SV40 Origin of Replication Activates Expression from SV40 Promoterless Constructs

Co-transfection with expression plasmids is widely used to control DNA uptake efficiency in transient transfection experiments. However, a number of problems have been associated with their use. Here, we describe the activation of expression of constructs not containing the simian virus 40 (SV40) or...

Full description

Bibliographic Details
Main Authors: A. Flint, S. Kaluz, M. Kaluzova, L. Sheldrick, P. Fisher, K. Derecka
Format: Article
Language:English
Published: Taylor & Francis Group 1999-10-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/99274st02