Co-Transfected SV40 Origin of Replication Activates Expression from SV40 Promoterless Constructs
Co-transfection with expression plasmids is widely used to control DNA uptake efficiency in transient transfection experiments. However, a number of problems have been associated with their use. Here, we describe the activation of expression of constructs not containing the simian virus 40 (SV40) or...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
Taylor & Francis Group
1999-10-01
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Series: | BioTechniques |
Online Access: | https://www.future-science.com/doi/10.2144/99274st02 |