Human NK Cell Up-regulation of CD69, HLA-DR, Interferon γ Secretion and Cytotoxic Activity by Plasmacytoid Dendritic Cells is Regulated through Overlapping but Different Pathways
Human plasmacytoid dendritic cells secrete high levels of IFNa and are thus implicated in the activation of NK cells. Activated NK cells are characterised by the up-regulation of CD69 and MHC class II DR expression, secretion of IFN g and enhanced cytotoxicity. We show that pDC mediate these process...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2009-01-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/9/1/386/ |
| _version_ | 1811303868139569152 |
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| author | Heather Donaghy George Rozis Martin Goodier Linda Klavinskis Frances Gotch Steven Patterson Adel Benlahrech |
| author_facet | Heather Donaghy George Rozis Martin Goodier Linda Klavinskis Frances Gotch Steven Patterson Adel Benlahrech |
| author_sort | Heather Donaghy |
| collection | DOAJ |
| description | Human plasmacytoid dendritic cells secrete high levels of IFNa and are thus implicated in the activation of NK cells. Activated NK cells are characterised by the up-regulation of CD69 and MHC class II DR expression, secretion of IFN g and enhanced cytotoxicity. We show that pDC mediate these processes by different mechanisms, some of which overlap. Human NK cells were analysed after co-culture with immature or CpG-matured blood pDC or with supernatant from these cells. Maximal CD69 expression by NK cells was mediated by supernatant from mature pDC and did not require pDC contact. Up-regulation was due in part to IFNa but also to factors in IFNa negative supernatant from immature DC. HLA-DR expression was independent of secreted molecules but required contact with immature or mature DC. Enhanced NK cytotoxicity, measured by killing of K562 targets and expression of CD107a, was mediated by multiple factors including type I IFN, supernatant from immature pDC cultures and contact with immature or mature pDC. These factors act cumulatively to enhance cytotoxcity. Thus different parameters of pDC mediated NK cell activation are regulated by distinct pathways. |
| first_indexed | 2024-04-13T07:55:53Z |
| format | Article |
| id | doaj.art-84869ffc6bd84f3794e13c5897224245 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-13T07:55:53Z |
| publishDate | 2009-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-84869ffc6bd84f3794e13c58972242452022-12-22T02:55:24ZengMDPI AGSensors1424-82202009-01-019138640310.3390/s90100386Human NK Cell Up-regulation of CD69, HLA-DR, Interferon γ Secretion and Cytotoxic Activity by Plasmacytoid Dendritic Cells is Regulated through Overlapping but Different PathwaysHeather DonaghyGeorge RozisMartin GoodierLinda KlavinskisFrances GotchSteven PattersonAdel BenlahrechHuman plasmacytoid dendritic cells secrete high levels of IFNa and are thus implicated in the activation of NK cells. Activated NK cells are characterised by the up-regulation of CD69 and MHC class II DR expression, secretion of IFN g and enhanced cytotoxicity. We show that pDC mediate these processes by different mechanisms, some of which overlap. Human NK cells were analysed after co-culture with immature or CpG-matured blood pDC or with supernatant from these cells. Maximal CD69 expression by NK cells was mediated by supernatant from mature pDC and did not require pDC contact. Up-regulation was due in part to IFNa but also to factors in IFNa negative supernatant from immature DC. HLA-DR expression was independent of secreted molecules but required contact with immature or mature DC. Enhanced NK cytotoxicity, measured by killing of K562 targets and expression of CD107a, was mediated by multiple factors including type I IFN, supernatant from immature pDC cultures and contact with immature or mature pDC. These factors act cumulatively to enhance cytotoxcity. Thus different parameters of pDC mediated NK cell activation are regulated by distinct pathways.http://www.mdpi.com/1424-8220/9/1/386/dendritic cellsNK cellscytokinescytotoxicity |
| spellingShingle | Heather Donaghy George Rozis Martin Goodier Linda Klavinskis Frances Gotch Steven Patterson Adel Benlahrech Human NK Cell Up-regulation of CD69, HLA-DR, Interferon γ Secretion and Cytotoxic Activity by Plasmacytoid Dendritic Cells is Regulated through Overlapping but Different Pathways Sensors dendritic cells NK cells cytokines cytotoxicity |
| title | Human NK Cell Up-regulation of CD69, HLA-DR, Interferon γ Secretion and Cytotoxic Activity by Plasmacytoid Dendritic Cells is Regulated through Overlapping but Different Pathways |
| title_full | Human NK Cell Up-regulation of CD69, HLA-DR, Interferon γ Secretion and Cytotoxic Activity by Plasmacytoid Dendritic Cells is Regulated through Overlapping but Different Pathways |
| title_fullStr | Human NK Cell Up-regulation of CD69, HLA-DR, Interferon γ Secretion and Cytotoxic Activity by Plasmacytoid Dendritic Cells is Regulated through Overlapping but Different Pathways |
| title_full_unstemmed | Human NK Cell Up-regulation of CD69, HLA-DR, Interferon γ Secretion and Cytotoxic Activity by Plasmacytoid Dendritic Cells is Regulated through Overlapping but Different Pathways |
| title_short | Human NK Cell Up-regulation of CD69, HLA-DR, Interferon γ Secretion and Cytotoxic Activity by Plasmacytoid Dendritic Cells is Regulated through Overlapping but Different Pathways |
| title_sort | human nk cell up regulation of cd69 hla dr interferon aza³ secretion and cytotoxic activity by plasmacytoid dendritic cells is regulated through overlapping but different pathways |
| topic | dendritic cells NK cells cytokines cytotoxicity |
| url | http://www.mdpi.com/1424-8220/9/1/386/ |
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