Effects of down-regulation of ackA expression by CRISPR-dCpf1 on succinic acid production in Actinobacillus succinogenes
Keypoints CRISPRi based on FnCpf1 and SpCas9opt was established in A. succinogenes. The target gene, ackA was repressed ~ eightfold by CRISPR-dCpf1. Repression of ackA increased succinate yield compared with ackA deletion.
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2023-01-01
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| Series: | AMB Express |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13568-023-01518-x |